Home \ Antibodies \ Macrophages mature, Human, mAb 25F9
Catalog # HM2158

Macrophages mature, Human, mAb 25F9

120,00333,00

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The monoclonal antibody 25F9 recognises a protein of 86 kD on the cell surface and within the cytoplasm of mature macrophages. The antibody is associated with fully differentiated tissue macrophages both in normal and in diseased tissues, independently of the presence or absence of inflammation. The antigen is absent on freshly isolated monocytes and other blood cells. After 6 to 7 days culture human monocytes become positive. Some melanoma and carcinoma cell lines are also positive. Furthermore the monoclonal antibody 25F9 cross reacts with a subpopulation of macrophages of rhesus monkey, pig alveolar macrophages and Kupffer cells. The monoclonal antibody 25F9 is very useful for macrophage phenotyping.

Application
Flow cytometry, Frozen sections, Paraffin sections, Western blot

Use
For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

Product type
Monoclonal antibodies
Quantity
20 µg, 100 µg
Formulation
0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Isotype
Mouse IgG1
Species
Human
Cross reactivity
Rhesus monkey – Yes
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease
Cardiology and metabolism, Infectious diseases

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