MARCO, Human, mAb PLK-1

Name: MARCO, Human, mAb PLK-1
SKU: HM2208
Availability: InStock

€133.00 – €8,873.00

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Description
Application

The monoclonal antibody PLK-1 recognizes the macrophage receptor with collagenous structure (MARCO). The scavenger receptors (SRs) expressed by macrophages are thought to play an important role in the immune response against bacteria by mediating ligand binding and phagocytosis. SRs can be divided into three different classes based upon their structural properties, which are termed SR-A, SR-B and SR-C. SRs-A are homotrimeric glycoproteins composed of 77 kDa monomers subdivided into 3 types.. The molecular structure of MARCO resembles that of SR-A type I, containing a triple-helical collagenous domain and a scavenger receptor cysteine-rich (SRCR) domain at the C terminus. MARCO is only expressed in some subpopulations of macrophages, although its expression can be strongly upregulated during infection or LPS treatment. Furthermore, MARCO is, like sinusoidal endothelial cell markers DC-SIGNR, LYVE-1 and stabilin-2, expressed by sinusoidal endothelial cells in lymph node. MARCO expressed by alveolar macrophages seems to play an important role in response to inhaled particles and airborne pathogens. The monoclonal antibody PLK-1 binds specifically to MARCO, and has been shown to partially block ligand binding.

Application

Flow cytometry, Frozen sections, Functional studies, Immuno fluorescence, Immuno precipitation, Western blot

Application Notes

IHC-F:- Tissue sections can be- fixed in acetone or 2% paraformaldehyde. PLK-1 antibody was used at 5 µg/ml (Ref. 3, 4).
FC: Antibody PLK-1 weakly stains alveolar macrophages by recognizing the extracellular domain of MARCO. Transfected COS cells were used as positive control (Ref. 1).
FS: Antibody PLK-1 blocks human alveolar macrophages binding to unopsonized particles (Ref.1,3,5).
IF: Aveolar macrophages and transfected CHO cells were stained for MARCO using 0.6 µg/ml PLK-1 antibody (Ref. 2, 5)
IP: Antibody PLK-1 immunoprecipitates MARCO as 60 and 50 kDa protein from lysates obtained from COS cells transfected with human MARCO (Ref. 3).
W: Antibody PLK-1 stained MARCO under non-reducing conditions (Ref. 6).

Use

For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For inhibition of biological activity in vitro dilutions have to be made according to the amounts of MARCO to be inactivated.

Positive Control

Human alveolar macrophages

Specifications
Downloads

Product type

Monoclonal antibodies

Amount

1 mg, 10 mg, 100 µg, 20 µg, 5 mg, 500 µg

Formulation

Quantities >500 µg will be send without preservative and carrier free. Quantities <500 µg will be send with preservative and carrier. exact formulation can be found on the datasheet.

Immunogen

Human alveolar macrophages (recognizes domain V between residues 420 and 431)

Isotype

Mouse IgG3

Species

Human

Cross reactivity

Bovine – Yes

Alias

SCARA2, scavenger receptor class A member 2, macrophage receptor with collagenous structure

Storage and stability

Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Precautions

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use or derivation of this product.

Disease

Infectious diseases

Protocol