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Catalog # HM2193

NGAL, Human, mAb 697


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The monoclonal antibody 697 reacts with human neutrophil lipocalin (HNL), also known as human nGAL (25 kDa). Lipocalins are a widespread family of small, robust proteins that typically transport or store biological compounds which are either of low solubility or are chemically sensitive, including vitamins, steroids, odorants and metabolic products. The members of this lipocalins share a high similarity in their tertiary structures in spite of a low degree in amino-acid sequence identity: three highly conserved sequence motifs form a funnel-like beta barrel which encloses a hydrophobic pocket for the internal ligand binding. The human neutrophil lipocalin (HNL) is produced by neutrophil leukocytes and stored in intracellular specific granules. Neutrophil lipocalin is covalently linked to gelatinase B (MMP-9) and proMMP is stored in three forms: the monomeric form (92 kDa), the homodimer (220 kDa) and a heterodimer (125 kDa). During inflammation and neoplastic transformation the human neutrophil lipocalin (HNL) is produced in neutrophils and in epithelial cells. Furthermore neutrophil lipocalin is a superior means to distinguish acute bacterial and viral infections as well as it enables the determination of the neutrophil activation level.

Immuno assays, Immuno fluorescence

For immunofluorescence dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
100 µg, 20 µg
0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Mouse IgG1
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Nephrology, Tumor immunology

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