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Catalog # HM1120

RBC, Mouse, mAb 34-3C

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The monoclonal antibody 34-3C recognizes an exposed surface determinant of intact red blood cells (RBC). The high-affinity anti-RBC monoclonal antibody efficiently bind to Fc receptors on macrophages inducing anemia in vivo due to a rapid Fc receptor (FcyR)-mediated erythrophagocytosis of opsonised RBC in spleen and livers. The capacity of the antibody to interact with FcyR is responsible for its haemolytic activity. The monoclonal antibody only recognises antigenic determinants expressed on Mouse RBC and not on other species of RBC.

Application
Flow cytometry, Functional studies

Use
For flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.

Positive Control
Mouse erythrocytes

Product type
Monoclonal antibodies
Amount
100 µg, 20 µg, 500 µg
Formulation
Quantities >500 µg will be send without preservative and carrier free. Quantities <500 µg will be send with preservative and carrier. exact formulation can be found on the datasheet.
Immunogen
RBC
Isotype
Mouse IgG2a
Species
Mouse
Cross reactivity
Chicken – No, Human – No, Rabbit – No, Rat – No, Sheep – No
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Autoimmunity, Infectious diseases
Protocol
Protocol Flow Cytometry
58 kb
CoA-TDS HM1067-HM2099
HM1120-100UG
53 kb
HM1120-20UG
53 kb
HM1120-500UG
53 kb
Safety Data Sheet
SDS - HM1120-100UG
51 kb
SDS - HM1120-20UG
51 kb
SDS - HM1120-500UG
50 kb

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