Home \ Antibodies \ S100A4, Human, mAb 1E2
Catalog # HM2326

S100A4, Human, mAb 1E2

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The monoclonal antibody clone 1E2 recognizes S100A4. S100A4 is a member of the S100 family. To date, the family consists of 22 members. S100 proteins are low molecular weight calcium binding proteins. The proteins consist of 2 calcium binding EF-hands located on the termini flanked by hydrophobic hinge regions. Calcium ions acts as a global second messenger involved in the regulation of many aspects of cell function. S100 proteins are involved in sensing and transforming calcium signals to downstream cellular responses. S100 proteins are located in the cytoplasm and/or nucleus of a wide range of cell types and involved in the regulation of cellular processes such as cell cycle progression and differentiation and inflammatory responses. Several S100 members have been found to act as cytokines in inflammation, particularly in autoimmune skin conditions such as psoriasis. S100A4 may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered S100A4 gene expression have been implicated in tumor metastasis. Studies have shown that S100A4 is involved tumor progression and metastasis in various malignant tumors. Detection of S100A4 expression becomes a promising candidate biomarker in cancer early diagnosis and prediction of cancer metastasis. Therefore S100A4 may have high therapeutic value.

Application
Functional studies, Immuno assays, Western blot

Application Notes
FS: Monoclonal antibody 1E2 blocks S100A4 angiogenic activity.
W: A reduced sample treatment and SDS-Page was used. The band size is 12 kDa.

Use
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.

Positive Control
A431 tumors

Product type
Monoclonal antibodies
Amount
100 µg, 20 µg
Formulation
0.2 ml (100 µg/ml) 0.2 µm filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin.
Immunogen
Full length recombinant human S100A4
Isotype
Mouse IgG1
Species
Human
Cross reactivity
Canine – Yes, Horse – Yes, Rabbit – Yes, Sheep – Yes
Alias
Protein S100-A4, Calvasculin, Metastasin, Placental calcium-binding protein, Protein Mts1, S100 calcium-binding protein A4</br> - Gene name: S100A4, CAPL, MTS1
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Tumor immunology
CoA-TDS HM2300-HM2400 Safety Data Sheet

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