Home \ Assays \ sTREM-1, Human, ELISA kit
Catalog # HK348

sTREM-1, Human, ELISA kit

748,001.232,00

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TREM-1 is member of a family of cell surface receptors which function as modulators of the inflammatory response in sepsis. TREM-1 is widely expressed on cells of the myeloid lineage and acts as an inflammatory trigger and amplifier after fungal and bacterial contact. Expression is predominantly seen on neutrophils, monocytes, macrophages, microglia, osteoclasts and dendritic cells. The TREM family consist of TREM-1, TREM-2, TREM-3 (mouse) and TREM-like transcript 1&2. They are members of the transmembrane immunoglobulin superfamily. TREM-1 is a 30 kD monomeric protein synthesized as a 234 amino acid (aa) precursor with a signal peptide (16 aa), an extracellular domain (184 aa), a transmembrane domain (29 aa), and a short cytoplasmic domain (5 aa). The ligand of TREM-1is unknown. The engagement of TREMs, after association with the adapter protein DAP12 (DNA activating protein 12) which contains an immunoreceptor tyrosine-based activation motif, triggers a signaling pathway that leads to intracellular calcium mobilization, actin cytoskeleton rearrangement, and activation of several transcription factors. TREM-1 acts in synergy with Toll-like receptor signaling pathways in amplifying the inflammatory response. During infections, receptor expression is modulated and soluble TREM-1 (sTREM-1, 17 kDa) is released. TREM-1 is shed from the membrane of activated phagocytes and can be found as sTREM-1 in tissue and body fluids like plasma and bronchoalveolar lavage fluid (BAL). Some studies suggest that sTREM1 is transcribed from an alternative mRNA. sTrem-1 is unable to transmit a signal but competes for binding with endogenous ligands. Thereby dampen the amplification loop which is activated when the infection is started. sTREM1 levels are possibly a prognostic marker for sepsis. However, some other studies show also elevated levels in non-infectious causes of inflammation (e.g. Crohn disease). At least sTREM-1 in biological fluids often correlates with severity of disease.

Application
The human sTREM-1 ELISA kit is to be used for the in vitro quantitative determination of human sTREM-1 in serum and plasma samples.

Principle
The human sTREM-1 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing human sTREM-1. Biotinylated tracer will bind to the captured human sTREM-1. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human sTREM-1 standards (log). The human sTREM-1 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.

Linearity
linearity of dilution of human plasma and serum samples for minimal 3 dilutions using a 2-fold dilution had a CV< 20%.

Recovery
Normal human blood samples (plasma) containing baseline levels of human sTREM-1, were mixed in three different ratios. Samples were measured using the ELISA. Recovery ranged from 88-98%.

Precision and reproducibility
Intra variation approved (CV < 20%) Inter variation approved (CV < 25%)

Product type
Assays
Quantity
1 x 96 det., 2 x 96 det.
Standard range
31 to 2000 pg/ml
Detection level
31 pg/ml
Working volume
100 µl/well
Species
Human
Cross reactivity
Canine – Weak, Goat – Weak, Horse – Yes, Mouse – Weak, Pig – No, Rabbit – No, Rat – No
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease
Autoimmunity, Infectious diseases
Safety Data Sheet Assay Manual
HK348
457 kb
HK348
356 kb

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