Home \ Antibodies \ Transgelin, Human, clone 4G4
Catalog # HM2405

Transgelin, Human, clone 4G4

Monoclonal antibody HM2405 clone 4G4 recognizes human transgelin, also called SM-22. Transgelin is considered to be a biomarker for acute intestinal ischemia (AII) and leiomyosarcoma.

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120,00375,00

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Monoclonal antibody HM2405 clone 4G4 recognizes human transgelin, also called SM-22. Transgelin is a 22 kDa calponin-like protein and exists as different isoforms a-d based on different pH. It is a water-soluble protein which is abundantly expressed by intestinal smooth muscle tissues. Its role is not complete clear but it seems to play a role in differentiation and maturation of smooth muscle cells. Transgelin is considered to be a biomarker for acute intestinal ischemia (AII) and leiomyosarcoma. AII is a relative rare abdominal emergency associated with considerable high morbidity and mortality as a consequence of obstruction of mesenteric blood flow associated with surgical procedures, hemorrhagic shock, trauma and sepsis. This leads to severe damage to the intestinal barrier and integrity. It is hypothesized that this transmural injury will lead to release of smooth muscle cell proteins like transgelin. Indeed, it has been shown that elevated levels of transgelin have been found in patients with transmural ischemia compared to controls.
The diagnosis of leiomyosarcoma is clinically important because it has been reported that soft tissue sarcomas showing a myoid differentiation, most often a smooth muscle differentiation, are more aggressive than those with no myoid differentiation. Transgelin correlates with smooth muscle differentiation. It was found that transgelin was one of the most represented in leiomyosarcomatous differentiation.

Application
Immuno assays, Paraffin sections, Western blot

Application Notes
IA: antibody 4G4 can be used as capture and detection antibody (Ref. 2)
IHC-P: For fixation 4% formaldehyde was used and blocking was done with 5% BSA in PBS (Ref.2)
W: Both reduced and non-reduced western blot can be used. The expected band size is 22 kDa (Ref.1)

Use
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
Quantity
20 µg, 100 µg
Formulation
0.2 µm filtered in PBS+0.1%BSA+0.02%NaN3
Immunogen
Purified SM22 from pig stomach
Isotype
Mouse IgG1
Species
Human
Alias
SM22, 22 kDa actin-binding protein, Protein WS3-10, Smooth muscle protein 22-alpha, SMCC, TAGLN1, WS3-10, SM22-alph
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Gastroenterology
CoA-TDS HM2300-HM2400

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