The monoclonal antibody 7-88 recognizes mouse Vascular Adhesion Protein-1 (VAP-1) which- is a glycosylated homodimeric membrane protein consisting of two 90 kDa subunits connected by disulfide bonds. It contains a short N-terminal cytoplasmic tail, a single membrane-spanning domain and a large extracellular part. A soluble form of VAP-1 (sVAP-1) has been described, which presumably results from the proteolytic cleavage of membrane-bound VAP-1. Structurally VAP-1 belongs to enzymes called semicarbamizide-sensitive amine oxidases, which contain copper as a cofactor. These enzymes deaminate primary amines in a reaction producing hydrogen peroxide, aldehyde, and ammonia.
VAP-1 is expressed in endothelial cells, smooth muscle cells, adipocytes, and in follicular dendritic cells. In endothelial cells the majority of VAP-1 is stored within intracellular granules and translocated to the surface upon inflammation where it regulates leukocyte tissue infiltration. Furthermore, the end-products formed by VAP-1 can also regulate leukocyte migration by signaling effects, have insulin-like effects in energy metabolism, and can cause vascular damage by direct cytotoxicity.
In- white adipose tissue of obese and diabetic db-/- mice increased expression of VAP-1 has been observed suggesting that it contributes- to the arthrosclerosis and vascular dysfunction observed in these diseases. Moreover, inhibition of VAP-1reduced the accumulation of myeloid cells into tumors and attenuates tumor growth.
The monoclonal antibody 7-88 inhibits migration of granulocytes and monocytes in acute models of inflammation.
Flow cytometry, Frozen sections, Functional studies, Immuno fluorescence, Immuno precipitation
FC: Antibody 7-88 stains the extracellular domain of mouse VAP-1 in CHO cells transfected with mouse VAP-1 cDNA. . As positive control- anti-VAP-1 clone 7-106 was used and as negative control an isotype-matched control antibody. (Ref. 2)
IHC-F:- Tissue sections were fixed in acetone and incubated with antibody 7-88 for 20 minutes at room temperature. . As negative control an irrelevant isotype-matched antibody was used (Ref.2).
FS: Antibody 7-88 (200 µm) was intravenously injected which resulted in the inhibition of leukocyte trafficking in inflamed peritoneum.- (Ref.2).
For immunohistochemistry and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, dilutions have to be optimized in user’s experimental setting.
Mouse VAP-1-transfected CHO cells (ref. 2)