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Description: Oxidized PAPC
|Formulation||1 mg dried OxPAPC prepared by air oxidation of synthetic PAPC according to ref. 1, packed under argon.|
|Use||1. For use of total amount in once: Add buffer or medium used in the experiment and resuspend lipids by vigorous vortexing for at least 30 seconds. Warm the vial up to 30°C and vortex again for 1 minute. Avoid preparing concentrated stocks since OxPAPC is poorly soluble in water. Sonicate if necessary to ensure better resuspension of OxPAPC, although that is usually not necessary at concentrations below 150 150μg/ml. The concentration range in which OxPAPC can be used depends on the cell type, but usually is below 100 μg/ml. High concentrations of OxPAPC can be toxic. For every new cell and assay type it is recommended to determine time- and concentration dependence.
2. For use of partial amount: Add chloroform to the vial to obtain lipid concentration of 1 to 10 mg/ml and carefully vortex avoiding contact of the solvent with vial cap. Aliquot OxPAPC solution into sterile glass (optimal) or polypropylene cell culture tubes. Before use check if the tubes are resistant to chloroform. Evaporate chloroform under a stream of nitrogen or argon gas with simultaneous vortexing in order to obtain a thin film of lipid on the tube walls. Resuspend in culture medium according to par. 1.
|Storage and stability||After arrival product should be stored at -20 °C or -70 °C. Under recommended storage conditions, product is stable for one year. Aliquots prepared in chloroform can be stored at preferably -70 °C for a few months.|
|References||1. Bochkov, V et al; Oxidized phospholipids stimulate tissue factor expression in human endothelial cells via activation of ERK/EGR-1 and Ca++/NFAT. Blood 2002, 99: 199
2. Birukov, K et al; Signal transduction pathways activated in human pulmonary endothelial cells by OxPAPC, a bioactive component of oxidized lipoproteins. Microvasc Res 2004, 67: 18
3. Zheng, M et al; Inhibition of LPS- and CpG DNA-induced TNF-alpha response by oxidized phospholipids. Am J Physiol Lung Cell Mol Physiol 2004, 286: L808
4. Birukov, K et al; Epoxycyclopentenone-containing oxidized phospholipids restore endothelial barrier function via Cdc42 and Rac. Circ Res 2004, 95: 892
5. Furnkranz, A et al; Oxidized phospholipids trigger atherogenic inflammation in murine arteries. Arterioscler Thromb Vasc Biol 2005, 25: 633
6. Blüml, S et al; Oxidized phospholipids negatively regulate dendritic cell maturation induced by TLRs and CD40. J Immunol 2005, 175: 501
|Precautions||For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.|
|Disease||Cardiology and metabolism, Tumor immunology|