0 items | $0.00

CpG-DNA, Mouse

Catalog #: HC4033

Quantity: 200 nmol

Availability: In stock

Description: CpG-DNA, Mouse

Bacterial DNA is known to induce acute inflammatory responses. Bacterial DNA acts as a pathogen-associated molecular pattern by virtue of a 20-fold greater frequency of unmethylated CG dinucleotides found in microbial DNA versus vertebrate DNA. Cellular activation by deoxy-cytidylate-phosphate-deoxy-guanylate (CpG)-DNA occurs via the Toll/IL-1R signal pathway. TLR9 appears to be a major component of the CpG-DNA receptor, acting by direct binding to CpG-DNA.
The synthetic oligodeoxynucleotides (ODN) contain the proper CpG-DNA motif mimicing the immunostimulatory effects of bacterial DNA. The human optimal CpG motif GTCGTT differs from the optimal mouse CpG motif GACGTT. Non CpG-DNA has been shown to compete with CpG-DNA in in vitro stimulation experiments. This mouse CpG is a 20-mer ODN has the following sequence: 5'-tccatgacgttcctgatgct-3'. Regular letters represent phosphorothiorate linkage.

Specifications

Catalog number HC4033
Product type LPS and ligands
Quantity 200 nmol
Species Mouse
Formulation Approximately 200 nmol lyophilized, purified 20-mer CpG ODN. The exact amount is indicated on the label. Reconstitute the vial by pipetting distilled or deionized water, volume depending on the concentration to be used.
Use It is recommended that users test the reagent and determine their own optimal concentrations.
Application FS
Application Notes FS: CpG-DNA can be used in biological assays in vitro to activate murine cells. For stimulation in vitro 0.01 to 10 μM can be used.
Storage and stability Lyophilized product should be stored at 4°C. Store stock solution in aliquots at -70°C. Repeated freeze and thaw cycles will cause loss of activity. Under recommended storage conditions, product is stable for one year.
References 1. Kawabata, T et al; Functional Alterations of Liver Innate Immunity of Mice with Aging in Response to CpG-Oligodeoxynucleotide. Hepatology 2008, 48:1586
2. Nakashima, H et al; Activation of CD11b+ Kupffer Cells/Macrophages as a Common Cause for Exacerbation of TNF/Fas-Ligand-Dependent Hepatitis in Hypercholesterolemic Mice. PLoSOne 2012, 8:e49339
3. Hou, X et al; CD205-TLR9-IL-12 axis contributes to CpG-induced oversensitive liver injury in HBsAg transgenic mice by promotin the interaction of NKT cells with Kupffer cells. Cell Mocl Immunol 2016, 13: 1
4. Yong, L et al; Identification of pro-inflammatory CD205+ macrophages in livers of hepatitis B virus transgenic mice and patients with chronic hepatitis B. nature Scien Rep 2017
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease Infectious diseases