C1-INH, Human, ELISA

Name: C1-INH, Human, ELISA
SKU: HK396
Availability: InStock

C1 Inhibitor (C1-INH), a central regulator of the complement system, plays a pivotal role in modulating immune response, with critical implications in both innate and adaptive immunity.

€756.00 – €1,223.00

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C1 Inhibitor (C1-INH) stands as a sentinel in immune regulation, orchestrating the delicate balance of our body’s defense mechanisms. As a master suppressor, C1-INH targets key enzymes across various immune pathways, safeguarding against unwarranted inflammation and preserving vascular stability. Its distinctive function in conserving the Alternative Pathway, while meticulously regulating the Classical and Lectin pathways, marks it as an integral component in immune homeostasis. In the clinical realm, C1-INH is pivotal in managing hereditary angioedema (HAE)—a condition marked by sudden, intense swelling. As a diagnostic marker, C1-INH is gaining recognition for its potential to detect early signs of MIS-C, SLE, and MS, heralding a new era in the proactive management of these complex conditions.

Application

The Human C1-INH ELISA kit is to be used for the in vitro quantitative determination of C1-INH in plasma and serum samples. This kit is intended for laboratory research use only and is not for use in diagnostic or therapeutic procedures. The analysis should be performed by trained laboratory professionals.

Principle

The Human C1-INH ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 1 hour and 15 minutes.

The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay.

Samples and standards are incubated in microtiter wells coated with antibodies recognizing Human C1-INH.

Peroxidase-conjugated antibody will bind to the captured C1-INH.

Peroxidase-conjugate will react with the substrate, tetramethylbenzidine (TMB).

The enzyme reaction is stopped by the addition of oxalic acid.

The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the Human C1-INH standards (log).

The Human C1-INH concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.

Precision and reproducibility

The intra assay and inter assay variation was tested and the intra variation was between 1.6 and 2.9%. The inter variation of the assay was between 1.3 and 4.1%.

Specifications
Downloads

Product type

Assays

Amount

1 x 96 det., 2 x 96 det.

Standard range

3.9 to 250 ng/ml

Detection level

3.9 ng/ml

Working volume

100 µl/well

Species

Human

Cross reactivity

Dog – No, Horse – No, Mouse – No, Pig – No, Rat – No

Storage and stability

Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.

Precautions

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.

Disease

Neurological disorders

Assay Manual