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Catalog # HP8012

C3, Mouse, pAb

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The complement factor C3 consists of an alpha- and a beta-chain. C3 is a central factor in the complement cascade. It is central to the alternative pathway that leads to the C3 convertase C3bBb. The classical and mannose binding lectin activation pathways lead to the C3 convertase C4b2a. These convertases cleave C3 resulting in C3a and C3b. Further degradation leads to formation of the alpha-chain products C3d, C3g and C3c. C3 is an acute phase protein that is produced by a wide range of tissues including renal epithelial cells and hepatocytes.

Application
Frozen sections, Immuno fluorescence, Paraffin sections, Western blot

Application Notes
F: Sections were permeabilized in cold acetone for 10 minutes.
P: Epitope retrieval for the FFPE sections was performed using the PT-module (Lab Vision) with heat induced epitope retrieval pH 9 (HIER).

Use
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Positive Control
Kidney, spleen

Product type
Polyclonal antibodies
Amount
100 µg, 20 µg
Formulation
0.2 ml (100 μg/ml) 0.2 μm filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Isotype
Rabbit Ig
Species
Mouse
Alias
Complement component C3, HSE-MSF</br> - Gene name: C3
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease
Infectious diseases, Nephrology
Protocol
Protocol Western Blot
59 kb
Protocol SDS Page
57 kb
Protocol IHC-F
36 kb
CoA-TDS pAb
HP8012
114 kb
HP8012-20UG
114 kb
Safety Data Sheet
SDS - HP8012-100UG
52 kb
SDS - HP8012-20UG
52 kb

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