C5, Human, ELISA

Name: C5, Human, ELISA
SKU: HK390
Availability: InStock

Complement C5 (molecular weight app. 190kDa) is a key component of the complement system.

€756.00 – €1,223.00

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C5, a key 190kDa protein in the complement system, is vital for both innate and adaptive immune responses. It spearheads protective actions against pathogens, paving the way for adaptive immunity. Present in blood, tissues, and body fluids, C5 forms part of the complex complement system. Its activation, through classical, lectin, and alternative pathways, leads to the formation of the Membrane Attack Complex (MAC). This complex is critical for neutralizing harmful microorganisms and damaged cells. Yet, uncontrolled C5 activation is linked to diseases like asthma, lupus, and multiple sclerosis. Genetic mutations causing C5 deficiency result in recurrent bacterial infections, highlighting its crucial role in immunity. C5’s importance extends beyond immune defense, positioning it as a target for treating various immune-related disorders. This underscores the significance of C5 in immune system function and disease management.

Application

The Human C5 ELISA kit is to be used for the in vitro quantitative determination of C5 in plasma and serum samples.

Principle

The Human C5 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours.
The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay.
Samples and standards are incubated in microtiter wells coated with antibodies recognizing Human C5.
Biotinylated tracer antibody will bind to the captured Human C5.
 Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody.  Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB).  The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the Human C5 standards (log).
The Human C5 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.

Recovery

Normal human blood samples (plasma) containing baseline levels of human C5, were spiked with human C5, in concentrations of 100 and 12.5 ng/ml. Samples with and without human C5, were incubated for 30 minutes at room temperature. Samples were measured using the ELISA. Values for human C5, ranged between 96% and 109%.

Specifications
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Product type

Assays

Amount

1 x 96 det., 2 x 96 det.

Standard range

3.1 to 200 ng/ml

Detection level

3.1 ng/ml

Working volume

100 µl/well

Species

Human

Cross reactivity

Cynomolgus monkey – Yes, Horse – No, Pig – No, Rat – No

Storage and stability

Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.

Precautions

Note that this user protocol is not lot-specific and is representative for the current specifications of this product. Please consult the vial label and the Certificate of Analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions. - For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.

Disease

Neurological disorders

Safety Data Sheet