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Catalog # HM1019

MCP-1, Mouse, mAb ECE.2


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The monoclonal antibody ECE.2 recognizes mouse monocyte chemoattractant protein 1 (MCP-1).
The murine JE gene encodes the monocyte-specific cytokine monocyte chemotactic protein 1 (MCP-
1). MCP-1 is a CC chemokine of 76 amino acids (~11 kDa) and is chemotactic for monocytes and
basophils but not neutrophils and eosinophils. MCP-1 is expressed by smooth muscle cells (SMC),
macrophages, endothelial cells, keratinocytes and fibroblasts in response to inflammatory stimuli such
as interleukin 1β and tumor necrosis factor α. MCP-1 has been implicated in a variety of inflammatory
processes, including inflammatory bowel disease, rheumatoid arthritis, asthma, nephritis, and parasitic
and viral infections. MCP-1 antigen is not detected in the endothelium or SMC of normal arteries.
MCP-1 has also been shown to exhibit biological activities other than chemotaxis. It can induce the
proliferation and activation of killer cells known as CHAK (CC-Chemokine-activated killer)
MCP-1 signals via the CCR2 receptor, and is critical for aneurysm formation because of its stability to
recruit leukocytes. These leukocytes produce extracellular matrix-degrading MMPs, thereby inductin
aortic remodelling and dilatation. Interleukin-6 is also involved in this amplification loop accelerating
vascular inflammation. MCP-/- mice display significantly delayed wound re-epithelialization, and also
delayed wound angiogenesis.

Frozen sections, Western blot

Application Notes
F: Sections (6 μm) were fixed with 4% PFA, blocked using 0.1% Triton-X and 5% serum for 15min at 37°C or o/n 4 °C and then incubated with 2 μg/ml antibody for 2h at 37°C.
W: Samples electrophoresed on 15% SDS-PAGE were blotted on nitrocellulose and blocked with PBS/5% low fat dry milk. The blot was incubated with antibody (0.8 μg/ml) for 20 min at RT.

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
100 µg, 20 µg
0.2 ml (100 μg/ml) 0.2 μm filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Synthetic peptide corresponding to residues 102-130 of mouse MCP-1
Rat IgG1
Cross reactivity
Human – No
C-C motif chemokine 2, Monocyte chemotactic and activating factor, Monocyte secretory protein JE, Small-inducible cytokine A2, Sigje </br> - Gene name: Ccl2
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Autoimmunity, Infectious diseases

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