Home \ Antibodies \ MPO, Rat, mAb 2D4, FITC
Catalog # HM3030F

MPO, Rat, mAb 2D4, FITC

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The monoclonal antibody 2D4 reacts with rat myeloperoxidase (MPO). MPO is a glycoprotein with an alpha2beta2 heteromultimer expressed in all cells of the myeloid linage. MPO is abundantly present in azurophilic granules of polymorphonuclear neutrophils. It is an important enzyme used during phagocytic lysis of engulfed foreign particles which takes part in the defense of the organism through production of hypochlorous acid (HOCl), a potent oxidant. MPO is rapidly released by activated polymorphonuclear neutrophils. Involvement of MPO has been described in numerous diseases such as atherosclerosis, lung cancer, Alzheimer’s disease and multiple sclerosis. Autoimmune antibodies to MPO are involved in Wegeners disease. Since the discovery of MPO deficiency, initially regarded as rare and restricted to patients suffering from severe infections, MPO has attracted more clinical attention. In experimental studies antibodies to MPO can be used for various purposes ranging from flow cytometric analysis to detection of polymorphonuclear neutrophils in tissue sections.

Application
Flow cytometry, Immuno assays

Application Notes
IA: the antibody can be used as a detection antibody.
FC: Cells are fixed with 4% paraformaldehyde and permeabilized with 0.1% saponine.

Use
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
Amount
100 µg, 20 µg
Formulation
0.2 ml (100 μg/ml) 0.2 μm filtered protein G purified FITC conjugated antibody solution in PBS, containing 1.0% bovine serum albumin and 0.02% sodium azide.
Conjugate
FITC
Immunogen
Mouse myeloperoxidase
Isotype
Mouse IgG1
Species
Rat
Cross reactivity
Mouse – Yes
Alias
Myeloperoxidase</br> - Gene name: Mpo
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease
Cardiology and metabolism, Pulmonology
Protocol
Protocol Sandwich ELISA
49 kb
Protocol Immuno Fluorescence
39 kb
Protocol Flow Cytometry
58 kb
Protocol IHC-F
36 kb
CoA-TDS HM3000
HM3030F
90 kb
HM3030F-20UG
90 kb
Safety Data Sheet
SDS - HM3030F-100UG
52 kb
SDS - HM3030F-20UG
52 kb

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