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TNF-alpha, Human, mAb 52B83-0
Catalog # HM2010

TNF-alpha, Human, mAb 52B83


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The monoclonal antibody 52B83 specifically targets tumor necrosis factor alpha (TNF-alpha), a crucial protein in immune responses and inflammation. TNF-alpha, a 17 kDa homotrimeric protein, interacts with TNF-receptors I and II, initiating varied cellular signaling pathways.

Initially recognized for its anti-tumor effects, TNF-alpha destroys tumor cells both in vivo and in vitro, causing tumor necrosis and demonstrating cytolytic activity. Beyond its role in tumor biology, TNF-alpha stands at the center of many inflammatory and immunological processes. It responds to microbial products and other cytokines by triggering a broad cytokine response, illustrating its key position in inflammation.

TNF-alpha’s pro-inflammatory actions are particularly significant in autoimmune diseases like rheumatoid arthritis. Neutralizing TNF-alpha has proven beneficial, offering therapeutic potential in such conditions.

The 52B83 antibody’s interaction with TNF-alpha opens new avenues for treatment in autoimmune diseases, highlighting the importance of targeted therapies. This insight reinforces TNF-alpha’s vital role in immunology and inflammation research.

Flow cytometry, Frozen sections, Immuno assays, Western blot

Application Notes
FC: Antibody 52B83 was used to stain soluble TNF bound to TNF receptors. Cells were fixed in PBA containing 0.2% formaldehyde (Ref. 3)
W: A reduced sample treatment and 15% SDS-Page was used. The band size observed is 17 kDa (Ref.4).
IHC-F: 6-μm tissue sections were fixed in acetone for 10 minutes (Ref. 4).

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
100 µg, 20 µg
0.2 ml (100 μg/ml) 0.2 μm filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Mouse IgG1
Cross reactivity
Guinea pig – Yes, Mouse – Yes, Rhesus monkey – Yes
Tumor Necrosis Factor-alpha, TNF, TNF-SF2, cachectin
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Autoimmunity, Gastroenterology

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