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Catalog # HM2406

Transgelin, Human, clone 1D2

Monoclonal antibody clone 1D2 recognizes human transgelin, also called SM-22.

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Monoclonal antibody clone 1D2 recognizes human transgelin, also called SM-22.

Transgelin is a 22 kDa calponin-like protein and exists as different isoforms a-d based on different pH. It is a water-soluble protein which is abundantly expressed by intestinal smooth muscle tissues. Its role is not complete clear but it seems to play a role in differentiation and maturation of smooth muscle cells.

Transgelin is considered to be a biomarker for acute intestinal ischemia (AII) and leiomyosarcoma. AII is a relative rare abdominal emergency associated with considerable high morbidity and mortality as a consequence of obstruction of mesenteric blood flow associated with surgical procedures, hemorrhagic shock, trauma and sepsis. This leads to severe damage to the intestinal barrier and integrity. It is hypothesized that this transmural injury will lead to release of smooth muscle cell proteins like transgelin. Indeed, it has been shown that elevated levels of transgelin have been found in patients with transmural ischemia compared to controls.

The diagnosis of leiomyosarcoma is clinically important because it has been reported that soft tissue sarcomas showing a myoid differentiation, most often a smooth muscle differentiation, are more aggressive than those with no myoid differentiation. Transgelin correlates with smooth muscle differentiation. It was found that transgelin was one of the most represented in leiomyosarcomatous differentiation.

Immuno assays, Immuno fluorescence, Paraffin sections, Western blot

Application Notes
IA: antibody 1D2 can be used as capture and detection antibody (Ref. 2) IHC-P: For fixation 4% formaldehyde was used and blocking was done with 5% BSA in PBS (Ref.2) W: Both reduced and non-reduced western blot can be used. The expected band size is 22 kDa (Ref.1)

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
100 µg, 20 µg
0.2 µm filtered in PBS+0.1%BSA+0.02%NaN3
Purified SM22 from pig stomach
Mouse IgG1
SM22, 22 kDa actin-binding protein, Protein WS3-10, Smooth muscle protein 22-alpha, SMCC, TAGLN1, WS3-10, SM22-alph
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Gene Name: TAGLN, Uniprot: Q01995, Entrez Gene ID: 6876
CoA-TDS HM2406 SDS HM2406
52 kb

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