FHR4, Human, pAb

Quantity:
20 µg
Catalog #:
HP9058-20UG

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Polyclonal antibody HP9058 recognizes human complement factor H related protein 4 (FHR4). The complement system is the first line of defense against pathogens and facilitates elimination of apoptotic and damaged cells. Complement factor H (fH) is an abundant plasma protein that negatively regulates the alternative pathway and the C3b amplification loop. The proteins encoded by the fH gene family includes besides fH also five fH-related proteins (FHR1-5), encoded by closely linked but unique genes. Like fH these proteins consist of repeating short consensus repeats (SCRs). fH competes with fB for binding to C3b and C3b(H2O) thereby blocking the formation of the convertase, accelerates decay of C3-convertases and is a cofactor for fI. The function of FHR proteins is less clear. FHRs are produced primarily in the liver and secreted in the plasma. FHR4 binds to the C3d region of C3b. In contrast to fH, the FHR4-C3b interaction promotes complement activation. Two transcripts of the FHR4 gene exist: FHR4B and FHR4B, consisting of respectively 9 and 5 SCRs (86& 42kDa). Both variants have different binding profiles to C3b. Suggested ligand in complement activation of FHR4 is pentameric CRP.
Specifications
Product type Polyclonal antibodies
Quantity 20 µg
Formulation 0.2 ml (100 μg/ml) 0.2 μm protein A purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Application Immuno assays, Western blot
Use Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Immunogen Recombinant FHR4 fragment SCR2-4
Isotype Rabbit Ig
Species Human
Alias Complement factor H-related protein 4, FHR-4
Gene name: CFHR4, CFHRL4, FHR4
References 1. Hebecker, M et al; Factor H-Related Protein 4 Activates Complement by Serving as a Platform for the Assembly of an Alternative Pathway C3 Convertase via its Interaction with C3b. J Biol Chem. 2012, 287:19528
Storage and stability Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease Autoimmunity, Nephrology
Applications
Application: Immuno assays, Western blot
Application notes: W: A non-reduced and/or reduced sample treatment and SDS-Page was used. The band size is 86 kDa.
IA: HP9058 can be used as detection antibody.
Use: Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
References
References: 1. Hebecker, M et al; Factor H-Related Protein 4 Activates Complement by Serving as a Platform for the Assembly of an Alternative Pathway C3 Convertase via its Interaction with C3b. J Biol Chem. 2012, 287:19528
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