Pentraxin 3, Human, ELISA kit

Catalog #: HK347-02

Quantity: 2 x 96 det.

Availability: In stock

Description: Pentraxin 3, Human, ELISA kit

Pentraxins are a superfamily of acute phase reactants charactarized by a pentameric structure. The C-reactive protein (CRP) and serum amyloid P-component (SAP) are wellcharactarized short Pentraxins, which are produced in the liver in response to inflammatory mediators. Pentraxin 3 (PTX3), also known as Tumor necrosis factor-Stimulated Gene 14 (TSG-14), is the prototypic long Pentraxin, with a high degree of conservation from mouse to man. Human PTX3 encodes a 45 kDa secretory glycoprotein with a 162 amino acid N-terminal extension and a 202 amino acid C-terminal Pentraxin domain. The protein is locally produced and released by a variety of cell types including macrophages, neutrophils, myeloid-derived mesangial cells, synovial cells, smooth muscle cells, alveolar epithelium, and glial cells. PTX3 is induced in response to either inflammatory cytokines interleukin-1 β (IL-1 β) and tumor necrosis factor α (TNFα) or the selected associated molecular patterns (PAMPs). PTX3 has a dual role in the regulation of the innate immune response. Via its C-terminal Pentraxin domain, immobilized PTX3 will bind complement component C1q to induce classical complement activation. However, fluid-phase PTX3 inhibits classical complement activation. Furthermore PTX3 participates in the clearance of apoptic cells. PTX3 is elevated in critically ill patients, with a gradient from systematic inflammatory resonse syndrome to septic shock, and in several other diseases, such as mycocardial infarction, rheumatoid arthritis, atherosclerosis, small vessel vasculitis and psoriasis. Furthermore serum levels of PTX3 are higher in preeclamsia compared with normal pregancies.

Specifications

Catalog number HK347-02
Product type Assays
Quantity 2 x 96 det.
Standard range 0.04 - 10 ng/ml
Detection level 0.04 ng/ml
Working volume 100 µl/well
Species Human
Alias Tumor necrosis factor Stimulated Gene 14 (TSG-14)
Application The human Pentraxin 3 ELISA kit is to be used for the in vitro quantitative determination of human Pentraxin 3 in plasma samples and cell culture supernatant.
Disease Cardiology and metabolism, Infectious diseases
Principle The human pentraxin 3 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are captured by a solid bound specific antibody. Biotinylated tracer antibody will bind to captured human pentraxin 3. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human pentraxin 3 standards (log). The human pentraxin 3 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.
Linearity The linearity of the assay was determined by serially diluting a sample containing 2353 pg/ml human pentraxin 3. The diluted samples were measured in the assay. The line obtained a slope of 1.005 and a correlation coefficient of 1.000.
Storage and stability Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
References 1. Peri, G. et al; PTX3, a prototypical long Pentraxin, is an early indicator of acute myocardial infarction in humans. Circulation 2000, 102: 636
2. Fazzini, F. et al; PTX3 in small-vessel vasculitides. An independent indicator of disease activity produced at sites of inflammation. Arthritis Rheum 2001, 44: 2841
3. Rovere-Querini, P. et al; Plasma and tissue expression of the long Pentraxin 3 during normal pregnancy and preeclampsia. Obstet Gynecol 2006, 108:149
4. Kanbay, M et al; Uric Acid and Pentraxin-3 Levels Are Independently Associated with Coronary Artery Disease Risk in Patients with Stage 2 and 3 Kidney Disease. Am J Nephrol 2011, 33: 325