MPO Rat ELISA Kit: Myeloperoxidase Analysis
MPO, a pivotal glycoprotein, showcases an alpha2beta2 heteromultimer structure and is integral in all myeloid lineage cells. This enzyme is notably concentrated in the azurophilic granules of polymorphonuclear neutrophils and plays a crucial role in the organism’s defense mechanisms. It accomplishes this by producing hypochlorous acid (HOCl), a powerful oxidant, during the phagocytic degradation of foreign entities.
The MPO Rat ELISA Kit brings precision to your research, especially in understanding MPO’s dynamic release by activated polymorphonuclear neutrophils. It scrutinizes the enzyme’s role outside the cell, where it catalyzes protein chlorination, evident in byproducts like 3-chlorotyrosine, and disrupts the functional integrity of crucial lipoproteins such as LDL and apolipoprotein A-I. These processes are instrumental in conditions like atherosclerosis, lung cancer, Alzheimer’s disease, and multiple sclerosis, marking MPO’s significance in a spectrum of pathologies.
Traditional MPO assays, primarily enzymatic, face limitations due to the presence of inhibitory substances in tissue homogenates and plasma, often yielding inconsistent results. The MPO Rat ELISA Kit transcends these challenges, offering reliability and precision unaffected by the enzyme’s inhibitors. This makes it an invaluable tool in both clinical and research settings, especially for conditions like Wegener’s disease, where autoimmune antibodies target MPO.
Elevate your research with the specificity and dependability of the MPO Rat ELISA Kit, designed to deliver unerring results even in complex analytical scenarios. Embrace the next level of MPO analysis and unlock new potentials in your scientific endeavors.
Perform curve fitting with Assayfit Pro with the online ELISA calculation sheets. Click here for the terms for use and distribution. Alternatively use the Excel add-in to perform data analysis
The rat MPO ELISA kit is to be used for the in vitro quantitative determination of rat MPO in plasma, tissue homogenate and cell culture supernatant samples.
The rat MPO ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours.
The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay.
Samples and standards are captured by a solid bound specific antibody.
Biotinylated tracer antibody will bind to captured rat MPO.
Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody.
Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB).
The enzyme reaction is stopped by the addition of citric acid.
The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the rat MPO standards (log).
The rat MPO concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.
The linearity of the assay was determined by serially diluting a serum sample containing 50 ng/ml rat MPO. The diluted samples were measured in the assay. The line obtained a slope of 0.90 and a correlation coefficient of 0.999.
Normal rat blood samples (plasma), were spiked with rat MPO in concentrations of 100 ng/ml. Samples with and without rat MPO were incubated for 1 hour at room temperature. Samples were measured using the ELISA. Recovery values for rat MPO ranged between 108% and 120% (mean 114 %).