sCD59, Human, ELISA kit

Catalog #: HK374-02

Quantity: 2 x 96 det.

CD59 (protectin) is a glycophosphoinositol (GPI)-anchored inhibitor of the membrane attack complex of complement found on blood cells, endothelia and epithelial cells. It is expressed on all leukocytes, erythrocytes and most other cell types.

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Description: sCD59, Human, ELISA kit

CD59 is a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. Complement activation ultimately leads to formation of the cytotoxic, poreforming membrane attack complex (MAC), the main effector of complement-mediated tissue damage. Insertion of the MAC into cell membranes induces the release of cytokines and growth factors that promote inflammation, thrombosis, and cell proliferation. CD59 is a complement regulatory protein ubiquitously expressed on mammalian cell surfaces and it specifically inhibits MAC formation and thereby protects “self” cells from complement-mediated damage. The protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in the CD59 gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. CD59 was demonstrated to be present in plasma/serum of healthy individuals in levels ranging from 22 to 119 ng/ml.

Specifications

Catalog number HK374-02
Product type Assays
Quantity 2 x 96 det.
Standard range Measurable concentration range of 0.39 to 25 ng/ml.
Detection level Minimum concentration which can be measured is 0.39 ng/ml.
Working volume 100 µl/well
Species Human
Cross reactivity Mouse - No, Pig - No, Rat - No
Alias CD59 glycoprotein, MAC-inhibitory protein, MAC-IP, membrane inhibitor of reactive lysis, MIRL, Protectin
Application The sCD59 ELISA kit is to be used for the in vitro quantitative determination of human sCD59 in plasma.
Disease Infectious diseases
Principle The sCD59 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing human sCD59. Biotinylated tracer antibody will bind to the captured human sCD59. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human sCD59 standards (log). The human sCD59 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.
Storage and stability Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
References - Budding, K et al; A promoter polymorphism in the CD59 complement regulatory protein gene in donor lungs correlates with a higher risk for chronic rejection after lung transplantation. Am J Transplant 2015, XX