Lipoteichoic acid, mAb 55
Catalog #: HM2048
Quantity: > 200 µg
The monoclonal antibody 55 recognizes lipoteichoic acid (LTA). LTA, a glycerol phosphate surface polymer, is a component of the envelope of Gram-positive bacteria. LTA is anchored via its glycolipids to the membrane and carries a polysaccharide chain extending into the peptidoglycan layer of the cell wall. LTA is released spontaneously into the culture medium during growth of gram-positive bacteria. LTA functions as an immune activator with characteristics very similar to lipopolysaccharide (LPS) from Gram-negative bacteria. LTA binds to CD14 and triggers activation predominantly via Toll-like receptor 2. Although LTA is internalized and traffics to the Golgi, the cellular activation in response to LTA occurs at the cell surface.
|Product type||Monoclonal antibodies|
|Quantity||> 200 µg|
|Formulation||1 ml (> 200 μg/ml) culture medium with a low endotoxin level containing 0.02% sodium azide.|
|Immunogen||Microbial mixture of Streptococcus sobrims HG961, HG962, HG970, and HG977 (Ref. 1)|
|Storage and stability||Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.|
|Precautions||For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.|
Application:F, FC, IA, IF, W
Application Notes:IA: For detection, 1.2 μg/ml antibody in PBS was added for 1 hr at 37 °C on LTA coated plates (Ref. 2,3,8). IF: 60’in PBS/0.02%BSA/0.02% saponin (ref 4) W: A reduced or native sample treatment and run on 15% SDS-Page. Blot was incubated o/n at 4°C with a 1/1000 dilution .The band size is ~17 kDa (Ref.6,7).
Positive Control:Culture medium of Gram-positive bacteria.
Negative Control:Culture medium of eukaryotic cells
Use:Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
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