Home \ Antibodies \ Lipoteichoic acid, mAb 55
Catalog # HM5018

Lipoteichoic acid, mAb 55

Read more
Please choose the quantity of items you wish to add to your cart…
Find distributor

The monoclonal antibody 55 recognizes lipoteichoic acid (LTA). LTA, a glycerol phosphate surface polymer, is a component of the envelope of Gram-positive bacteria. LTA is anchored via its glycolipids to the membrane and carries a polysaccharide chain extending into the peptidoglycan layer of the cell wall. LTA is released spontaneously into the culture medium during growth of gram-positive bacteria. LTA functions as an immune activator with characteristics very similar to lipopolysaccharide (LPS) from Gram-negative bacteria. LTA binds to CD14 and triggers activation predominantly via Toll-like receptor 2. Although LTA is internalized and traffics to the Golgi, the cellular activation in response to LTA occurs at the cell surface.

Application
Flow cytometry, Frozen sections, Immuno assays, Immuno fluorescence, Western blot

Application Notes
IA: For detection, 1.2  µg/ml antibody in PBS- was added for 1 hr at 37 °C on LTA coated plates (Ref. 2,3,8).
IF: 60' in PBS/0.02%BSA/0.02% saponin (ref 4)
W: A reduced or native sample treatment and run on 15% SDS-Page. Blot was incubated o/n at 4 °C with a 1/1000 dilution .The band size is ~17 kDa (Ref.6,7).

Positive Control
Culture medium of Gram-positive bacteria

Negative Control
Culture medium of eukaryotic cells

Product type
Monoclonal antibodies
Amount
100 µg, 20 µg
Formulation
0.2 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Immunogen
Microbial mixture of Streptococcus sobrims HG961, HG962, HG970 and HG977
Isotype
Mouse IgG3
Species
N/A
Alias
LTA
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Infectious diseases
CoA-TDS
HM5018-20UG
103 kb
HM5018
103 kb
Safety Data Sheet
SDS - HM5018-20UG
52 kb
SDS - HM5018-100UG
52 kb

You may also like…

Calculate your ELISA data easily

With the ELISA calculator you can easily calculate ELISA data. Assayfit Pro helps to perform curve fitting. The calculator generates advanced reports, fit graph, fit parameters and goodness of fit are shown.

Show data entry

Latest Hycult Biotech news

  • New Human Complement Pathway Assays
    We are very proud of our newly developed human classical and alternative complement pathway assays. They are produced in response to a growing demand for quantitative investigation of complement inhibitors or regulators at lower sample dilutions. This development aims to address the issue of false negative results, enabling more accurate and reliable analysis of complement… Read more: New Human Complement Pathway Assays
  • Navigating the pitfalls in complement analysis
    Our colleague Erik Toonen shared his experience on how to analyze complement at the Complement-based Drug Development Summit in Boston in September 11-13th, 2023. He showed valuable insights on analyzing complements. For accurate complement analysis, it is important that not only the correct technique is used but also that pre-analytical sample handling is performed in… Read more: Navigating the pitfalls in complement analysis
  • NEW Human C3d ELISA
    We are happy to introduce our new ELISA (cat.# HK3017) designed to detect human C3d. What sets this assay apart is its utilization of a distinctive neo-epitope capture antibody that selectively targets a specific C3d region of the alpha chain, which is not available in other C3 variants. This way it specifically distinguishes C3d from native C3… Read more: NEW Human C3d ELISA
Contact us