Lipoteichoic acid, mAb 55

Name: Lipoteichoic acid, mAb 55
SKU: HM5018
Availability: InStock

€125.00 – €481.00

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Description
Application

The monoclonal antibody 55 recognizes lipoteichoic acid (LTA). LTA, a glycerol phosphate surface polymer, is a component of the envelope of Gram-positive bacteria. LTA is anchored via its glycolipids to the membrane and carries a polysaccharide chain extending into the peptidoglycan layer of the cell wall. LTA is released spontaneously into the culture medium during growth of gram-positive bacteria. LTA functions as an immune activator with characteristics very similar to lipopolysaccharide (LPS) from Gram-negative bacteria. LTA binds to CD14 and triggers activation predominantly via Toll-like receptor 2. Although LTA is internalized and traffics to the Golgi, the cellular activation in response to LTA occurs at the cell surface.

Application

Flow cytometry, Frozen sections, Immuno assays, Immuno fluorescence, Western blot

Application Notes

IA: For detection, 1.2 µg/ml antibody in PBS- was added for 1 hr at 37 °C on LTA coated plates (Ref. 2,3,8).
IF: 60' in PBS/0.02%BSA/0.02% saponin (ref 4)
W: A reduced or native sample treatment and run on 15% SDS-Page. Blot was incubated o/n at 4 °C with a 1/1000 dilution .The band size is ~17 kDa (Ref.6,7).

Positive Control

Culture medium of Gram-positive bacteria

Negative Control

Culture medium of eukaryotic cells

Specifications
Downloads

Product type

Monoclonal antibodies

Amount

100 µg, 20 µg

Formulation

0.2 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.

Immunogen

Microbial mixture of Streptococcus sobrims HG961, HG962, HG970 and HG977

Isotype

Mouse IgG3

Species

N/A

Alias

LTA

Storage and stability

Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.

Precautions

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.

Disease

Infectious diseases

CoA-TDS