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Catalog # HM6011

Lipopolysaccharide Core, mAb WN1 222-5

The mouse monoclonal antibody clone WN1 222-5 recognizes the core region of lipopolysaccharide (LPS), whereas it lacks reactivity with free lipid A and Rd2 or smaller LPS.

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Meet the remarkable mouse monoclonal antibody clone WN1 222-5, a precision tool in the realm of immunology. This antibody has a unique ability – it targets the core region of lipopolysaccharide (LPS) with extraordinary accuracy while showing no reactivity to free lipid A, Rd2, or smaller LPS variants. Let’s delve into the intriguing world of LPS and its significance.

Lipopolysaccharide (LPS) is a key element of Gram-negative bacteria, infamous for causing infections via strains like E. coli and Salmonella. Comprising three parts—lipid A, core oligosaccharide, and O-polysaccharide—LPS forms the endotoxin essential to bacterial survival and virulence. Understanding the structure of LPS, especially the toxic lipid A and the variable O-polysaccharide, is critical for developing targeted antibacterial treatments.

Humans and vertebrates encounter LPS through exposure to enterobacteria, which release small amounts of endotoxins as they grow. LPS is heat-stable and can cause watery diarrhea and inflammation upon interacting with the digestive tract.

Additionally, LPS also plays a role in conditions like necrotizing enterocolitis (NEC), primarily affecting newborns. If LPS enters the bloodstream, it binds to host cells, triggering complex responses and, in severe cases, organ failure (septic shock).

Furthermore, LPS  inhibits Interleukin-6 (IL-6), which presents a strategic method for fine-tuning the body’s immune response. As a central cytokine, IL-6 is instrumental in both inflammation control and muscle function, serving a dual role in defense mechanisms and metabolism. Targeting IL-6 inhibition offers a pathway to mitigate conditions characterized by chronic inflammation, such as autoimmune disorders, metabolic syndromes, and certain types of cancers.

Functional studies, Immuno assays, Paraffin sections, Western blot

Application Notes
W: A non-reduced sample treatment and SDS-PAGE was used (Ref. 1 and 5).
IHC-P: Antigen retrieval was performed by heating sections in 1x DIVA Decloaker reagent. As positive control gut or lymph node of SIV-infected rhesus macaques was used and as negative control gut or lymph node of non-SIV-infected rhesus macaques (Ref.6).

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Positive Control
LPS E.coli

Negative Control
Normal lymph node (non-infected)

Product type
Monoclonal antibodies
1 mg, 10 mg, 100 µg, 20 µg, 5 mg, 500 µg
Quantities >500 µg will be send without preservative and carrier free. Quantities <500 µg will be send with preservative and carrier. exact formulation can be found on the datasheet.
10E8 heat-killed bacteria
Mouse IgG2a
LPS core
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Infectious diseases

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