Home \ Antibodies \ C1-INH, Human, clone 15/12
W: Non-reduced (NR) and reduced (R) western blot with antibody 15/12. Samples loaded were serum (ser): 0.2 µg/sample and purified C1-INH (C1-I), 0.3 µg/sample.
Catalog # HM2411

C1-INH, Human, clone 15/12

Mouse monoclonal antibody HM2411 recognizes human C1-inhibitor. C1-INH plays an important role in suppression of inflammation and vascular permeability.

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120,00375,00

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Mouse monoclonal antibody HM2411 recognizes human C1-inhibitor. The complement system plays important roles in both innate and adaptive immune response and can produce an inflammatory and protective reaction to challenges from pathogens before an adaptive response can occur. There are three pathways of complement activation. The classical pathway (CP) is initiated by Immune complexes; the lectin pathway (LP) by surface bound mannan binding lectin; and the alternative (AP) by all the surfaces that are not specifically protected against it. Each generates a C3 convertase, a serine protease that cleaves the central complement protein C3, and generates the major cleavage fragment C3b. The C3 and C5 convertases are enzymatic complexes that initiate and amplify the activity of the complement pathways and ultimately generate the cytolytic MAC (C5b-9). C1 inhibitor (C1-INH) is a heavily glycosylated single chain molecule of 500 AA. It inhibits multiple enzymes, including C1s&r of the CP and MASP-1&2 of the LP, plasmin in the fibrinolytic system and Factor XIIa&XIa of the contact and coagulation system. C1-INH is also called C1 esterase inhibitor, due C1s is often cleaved by synthetic esters in spectrophotometry. C1-INH plays an important role in suppression of inflammation and vascular permeability. C1-INH binding of C1 to the catalytic site of both C1r and C1s releases the latter two from the complex. As a result the activation of the complement system is blocked. Binding to MASP blocks function and thereby consumption of C2,3&4. C1-INH spares the AP, leaving part of the innate antibacterial defense intact. Besides, C1-INH can directly bind and neutralize LPS, inhibiting sepsis and endotoxin shock. C1-INH administration is the common treatment for hereditary angioedema (HAE). A disease commonly caused by heterozygous deficiency of C1-INH and leading to low levels of functional C1-INH and recurrent episodes of dermal and submucosal swelling. This is mediated by its ability to control activation of the contact system in inhibiting bradykinin generation and thereby control of vascular permeability.

Application
Immuno assays, Western blot

Application Notes
IA: Antibody 15/12 can be used as capture and detection antibody.
W: Antibody 15/12 recognizes C1-INH and the complex C1s-C1INH in western blot, both under reduced and non-reduced conditions. The expected band size is 100 kDa for C1-INH and 150 - 196 kDa for the complex.

Product type
Monoclonal antibodies
Quantity
20 µg, 100 µg
Formulation
0.2 µm filtered in PBS+0.1%BSA+0.02%NaN3
Immunogen
Human plasma derived C1-inhibitor purified by anion exchange chromatography, >99% pure
Isotype
Mouse IgG2a
Species
Human
Alias
EDSPD2
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
CoA-TDS HM2300-HM2400

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