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Catalog # HK366

C3, Human, ELISA kit


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C3, a central component in the complement system, plays a crucial role in both innate and adaptive immune responses. It acts as a frontline defense against pathogens, triggering inflammatory and protective reactions. Found in circulation, tissues, and body fluids, C3 is part of a complex protein and receptor family. Activation occurs via three pathways: the classical pathway, triggered by immune complexes; the lectin pathway, initiated by surface-bound lectins; and the alternative pathway (AP), activated by unprotected surfaces. These pathways converge to produce C3 convertase, which cleaves C3 into C3b, a major fragment. This process escalates into the formation of the membrane attack complex (MAC).

C3 synthesis varies across tissues, responding to diverse stimuli. Its cleavage results in anaphylotoxin C3a and C3b, the latter kickstarting the terminal complement pathway. Weighing around 185kDa, C3 stands as the most abundant complement protein, primarily produced by the liver and also by macrophages, neutrophils, and endothelial and epithelial cells.

C3’s high concentration and swift response to threats like pathogens make it a potent immune component. Deficiencies in C3 can lead to recurrent infections and autoimmune diseases. Although rare, C3 deficiency cases involve recurrent infections and impaired immune cell development. C3 polymorphism is linked to age-related macular degeneration (AMD) and atypical hemolytic uremic syndrome (aHUS). Furthermore, C3 assists in removing immune complexes, enhancing macrophage phagocytic capabilities. Dysfunctions in this system can lead to autoimmune diseases and tissue complement deposition, underscoring C3’s pivotal role in immune regulation.

The Human C3 ELISA kit is to be used for the in vitro quantitative determination of C3 in plasma and serum samples.

The Human C3 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are captured by a solid bound specific antibody. Biotinylated tracer antibody will bind to captured human C3. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the C3 standards (log). The human C3 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.

Product type
1 x 96 det., 2 x 96 det.
Standard range
1.6-100 ng/ml
Detection level
1.6 ng/ml
Working volume
100 µl/well
Cross reactivity
Cynomolgus monkey – Yes, Mouse – No, Pig – No, Rat – No
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Safety Data Sheet Assay Manual
392 kb

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