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Catalog # HK3017

C3d, Human, ELISA kit

C3d, a pivotal fragment of the complement protein C3, plays a crucial role in the body’s innate and adaptive immune responses.

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C3d, a critical fragment of complement protein C3, significantly impacts innate and adaptive immunity. This system, encompassing a diverse array of proteins and receptors in the body’s fluids and tissues, activates through three pathways: classical, lectin, and alternative. Each pathway generates a C3 convertase, splitting C3 into C3b and the anaphylatoxin C3a. C3b’s further breakdown on cell surfaces kickstarts the terminal complement pathway, creating C3d.

As the most abundant complement system protein, C3 has a molecular weight around 185kDa. C3d, at 38.9 KDa and non-glycosylated, emerges from C3b’s continual cleavage. It’s crucial in immune regulation, especially via interactions with complement receptor 2 (CR2). This role is essential in conditions like transplant rejection, kidney diseases, AMD, and various inflammatory diseases. C3d’s diagnostic value lies in its ability to indicate active complement activation, offering a more precise reflection than C3 and C4 levels. Despite the challenge that most antibodies can’t differentiate between C3d’s active and native states, its importance in the immune system and as a disease marker remains clear.

The Human C3d ELISA kit is to be used for the in vitro quantitative determination of C3d in plasma and serum samples.

The Human C3d ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 1 hour and 15 minutes. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing Human C3d. Peroxidase-conjugated antibody will bind to the captured C3d. Peroxidase-conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the Human C3d standards (log). The Human C3d concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.

Product type
1 x 96 det., 2 x 96 det.
Standard range
0.2 – 15 ng/ml
Detection level
0.2 ng/ml
Working volume
100 µl/well
Cross reactivity
Horse – No, Mouse – No, Pig – No, Rat – No
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Safety Data Sheet Assay Manual
402 kb

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