C3c, Human, ELISA kit

The human C3c ELISA kit is to be used for the in vitro quantitative determination of human C3c in serum and plasma.

The human C3c ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing human C3c. Biotinylated tracer antibody will bind to the captured human C3c. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human C3c standards (log). The human C3c concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.

The linearity of the assay was determined by serially diluting a sample containing human C1c. The diluted samples were measured in the assay.

Recovery was determined by mixing citrate AF003 plasma and M/66427 in a ratio of 75%/25%, 50%/50%, 25%/75% and 0%/100%. C3c was measured with capture HM2318 3 µg/ml. The recovery of the samples is between the acceptable values of 80-120%.