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Catalog # HK368

C3c, Human, ELISA kit

The C3c ELISA, designed for specificity to C3c, offers a direct approach for evaluating complement activation in inflammatory diseases, cementing C3c’s position as a crucial biomarker in immune system research.

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C3c, a key component in the complement system, plays a vital role in the body’s defense against infections and in regulating immune responses. Unlike other complement biomarkers, C3c stands out for its stability, not binding to pathogens or cell receptors, and thereby ensuring accurate assessment of immune activity. This makes C3c an invaluable marker for detecting inflammation and potential autoimmune disorders like HUS. Its association with cardiovascular health and Parkinson’s disease further elevates its utility.

The human C3c ELISA kit is to be used for the in vitro quantitative determination of human C3c in serum and plasma.

The human C3c ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing human C3c. Biotinylated tracer antibody will bind to the captured human C3c. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human C3c standards (log). The human C3c concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.

The linearity of the assay was determined by serially diluting a sample containing human C1c. The diluted samples were measured in the assay.

Recovery was determined by mixing citrate AF003 plasma and M/66427 in a ratio of 75%/25%, 50%/50%, 25%/75% and 0%/100%. C3c was measured with capture HM2318 3 µg/ml. The recovery of the samples is between the acceptable values of 80-120%.

Product type
1 x 96 det., 2 x 96 det.
Standard range
1.6 to 100 ng/ml
Detection level
1.6 ng/ml
Working volume
100 µl/well
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Infectious diseases, Nephrology
Cross reactivity
Cynomolgus monkey – Yes, Mouse – No, Pig – No, Rabbit – No, Rat – No
Safety Data Sheet Assay Manual
398 kb

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