Catalog # HK217
C4, Mouse, ELISA kit
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Application
The Mouse C4 ELISA kit is to be used for the in vitro quantitative determination of C4 in plasma samples. This kit is intended for laboratory research use only and is not for use in diagnostic or therapeutic procedures.
The analysis should be performed by trained laboratory professionals.
Principle
The Mouse C4 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 1 hour and 15 minutes.
The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay.
Samples and standards are incubated in microtiter wells coated with antibodies recognizing Mouse C4.
Peroxidase-conjugated antibody will bind to the captured C4.
Peroxidase-conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid.
The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the Mouse C4 standards (log).
The Mouse C4 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.
The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay.
Samples and standards are incubated in microtiter wells coated with antibodies recognizing Mouse C4.
Peroxidase-conjugated antibody will bind to the captured C4.
Peroxidase-conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid.
The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the Mouse C4 standards (log).
The Mouse C4 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.
Product type
Assays
Amount
1 x 96 det., 2 x 96 det.
Standard range
0.8 to 50 AU/ml
Detection level
0.8 AU/ml
Working volume
100 µl/well
Species
Mouse
Cross reactivity
Dog – No, Horse – No, Human – No, Pig – No, Rat – No
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.
Precautions
Note that this user protocol is not lot-specific and is representative for the current specifications of this product. Please consult the vial label and the Certificate of Analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions. -
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Assay Manual
Safety Data Sheet
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