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Box of Hycult Biotech ELISA kit
Catalog # HK3490

COMING SOON: C5a, Human, ELISA kit

The human C5a ELISA kit is to be used for the in vitro quantitative determination of C5a in serum and plasma samples.

€756.00 €1,223.00Price range: €756.00 through €1,223.00

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Application
Immuno assays

Use
The human C5a ELISA kit is to be used for the in vitro quantitative determination of C5a in serum and plasma samples.

Principle
The human C5a ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 1 hour and 15 minutes. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing human C5a. Peroxidase-conjugated antibody will bind to the captured C5a. Peroxidase-conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human C5a standards (log). The human C5a concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.

Amount
1 x 96 det., 2 x 96 det.
Product type
Assays
Working volume
100 µl/well
Species
Human
Cross reactivity
Cynomolgus monkey – Yes
Disease
Autoimmunity, Infectious diseases
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.

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