C5aR, Human, mAb S5/1
The monoclonal antibody S5/1 recognizes the human receptor for C5a (CD88). The C5a receptor- is a seven transmembrane GTP-binding-protein-coupled receptor of 45kDa which binds with high affinity to human anaphylatoxin C5a. Upon C5a binding- G-protein-dependent cellular- responses are initiated such as increased intracellular Ca2+, granule fusion with the cell membrane, enzyme release and oxidative burst suggesting that C5aR is an important mediator of inflammation. The C5a receptor is expressed on a broad range of cell types, including- neutrophils, monocytes/macrophages, dendritic cells, astrocytes and microglia.
Interestingly, C5a receptor signalling has been associated with several inflammatory diseases including rheumatoid arthritis.-
Clone S5/1 was raised against a synthetic peptide comprising the N-terminal domain of the C5aR (Met1-Asn31). The antibody has been shown to inhibit the binding of C5a to its receptor.
FS: Antibody S5/1 inhibits C5a binding to its receptor at a concentration of 3.3 µM (Ref. 2)
IHC-P: Tissue sections were fixed in 2% paraformaldehyde and pretreated with 5% normal rabbit serum before applying antibody S5/1 (Ref. 3).
W: extracts from smooth muscle cells were separated by SDS-PAGE, the observed band size with S5/1 antibody is 42kDa (Ref. 5).
Calculate your ELISA data easily
With the ELISA calculator you can easily calculate ELISA data. Assayfit Pro helps to perform curve fitting. The calculator generates advanced reports, fit graph, fit parameters and goodness of fit are shown.
We are glad to support you!
Our technical support team of qualified scientists is more than willing to assist.