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Catalog # HK503

LBP, various species, ELISA kit

LBP, an essential liver-produced protein, activates and neutralizes immune responses, highlighting its significance in immunological research.

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The liver constitutively produces Lipopolysaccharide (LPS) Binding Protein (LBP), a type 1 acute phase protein that swiftly increases during acute phase responses. LBP assumes a pivotal role in responding to LPS by catalyzing LPS monomerization and facilitating its transfer to (s)CD14 and lipoproteins. This dual function positions LBP at the heart of LPS’s activation pathway—triggering monocyte activation and the subsequent release of inflammatory mediators—and its neutralization pathway—enabling the lipoprotein-mediated uptake and clearance of LPS. Researchers widely use assays to measure functional LBP levels in cell culture supernatants, plasma, and serum from various species, noting its presence escalates in the plasma of healthy individuals during acute phase responses. This highlights LBP’s significance in both activating immune responses to pathogens and mitigating their potentially harmful effects, underscoring its critical role in immunology research.

The LBP ELISA kit for a wide variety of species is to be used for the in vitro quantitative determination of LBP in cell culture supernatants, plasma or serum.

The LBP ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of 1 plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibo­dies recognizing LBP. Biotinylated LPS will bind to captured LBP. Streptavidin-peroxidase conjugate will bind to the biotinylated LPS. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of citric acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the LBP standards (log). The LBP concentration of samples, which are run concurrently with the human LBP standards, can be determined from the human LBP standard curve.

Product type
1 x 96 det.
Standard range
1.6 to 100 ng/ml
Detection level
1.6 ng/ml
Working volume
100 µl/well
Cross reactivity
Bovine – Yes, Canine – Yes, Chimpanzee – Yes, Cynomolgus monkey – Yes, Human – Yes, Rabbit – Yes, Rat – Yes, Rhesus monkey – Yes, Sheep – Yes, Swine – Yes
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Infectious diseases
Assay Manual
499 kb
Safety Data Sheet

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