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Catalog # HK503

LBP, various species, ELISA kit

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Lipopolysacharide (LPS) Binding Protein (LBP) is a type 1 acute phase protein that is constitutively produced by the liver and rapidly upregulated during the acute phase response. LBP plays a central role in the response to LPS. The protein catalyses the monomerization of LPS and its transfer to (s)CD14 and to lipoproteins. In this way LBP has both a role in the activation pathway of LPS: activation of monocytes by LPS leading to release of inflammatory mediators and in the neutralization of LPS i.e. the uptake of LPS by lipoprotein and subsequent clearing. The assay can be used to quantify functional LBP in tissue culture supernatants of cell cultures, plasma and serum samples of several species. In plasma of healthy individuals LBP is present at levels which increase during acute phase responses.

Application
The LBP ELISA kit for a wide variety of species is to be used for the in vitro quantitative determination of LBP in cell culture supernatants, plasma or serum.

Principle
The LBP ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of 1 plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibo­dies recognizing LBP. Biotinylated LPS will bind to captured LBP. Streptavidin-peroxidase conjugate will bind to the biotinylated LPS. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of citric acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the LBP standards (log). The LBP concentration of samples, which are run concurrently with the human LBP standards, can be determined from the human LBP standard curve.

Product type
Assays
Quantity
1 x 96 det.
Standard range
1.6 to 100 ng/ml
Detection level
1.6 ng/ml
Working volume
100 µl/well
Species
N/A
Cross reactivity
Bovine – Yes, Canine – Yes, Chimpanzee – Yes, Cynomolgus monkey – Yes, Human – Yes, Rabbit – Yes, Rat – Yes, Rhesus monkey – Yes, Sheep – Yes, Swine – Yes
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease
Infectious diseases
Safety Data Sheet Assay Manual
HK503
439 kb

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