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Catalog # HK504-AGM

EndoCAb®, Human, ELISA kit

The EndoCAb® ELISA has been developed for determination of endotoxin core antibodies in human plasma or serum in patients or healthy individuals

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The HK504-AGM contains three EndoCAb® ELISA kits:
EndoCAb® IgA, Human, ELISA kit
EndoCAb® IgG, Human, ELISA kit
EndoCAb® IgM, Human, ELISA kit

The EndoCAb® ELISA has been developed for determination of endotoxin core antibodies in human plasma or serum in patients or healthy individuals. Several studies show a consistent drop in postoperative levels of circulating anti-endotoxin core antibodies from the preoperative value. This drop has been interpreted as consumption of antibodies to endotoxin by systemic release of endotoxin.

A hypothesis is that if the patients pre-operative EndoCAb® level is low, even moderately low, patients may not be able to cope with the efflux of endotoxin, which may have mild to severe clinical consequences. The assay is of interest for various experimental conditions ranging from in vitro LPS neutralization by plasma components to in vivo studies on kinetics of antibodies to endotoxin in health and diseases.

The EndoCAb® standard median-units IgG, IgM and IgA (MU) are arbitrary and are based on medians of ranges for 1000 healthy adults in a particular locality. It has not been established whether normal EndoCAb® values vary by region, culture or race. Users should establish appropriate local statistical controls for their studies. It is advised that studies of any patient group should always be controlled by studies of appropriately selected contrasting clinical groups and/or healthy individuals recruited locally. EndoCAb® is a registered trademark. Used under license from the Scottish National Blood Transfusion Service.

The EndoCAb® ELISA kit is to be used for the in vitro quantitative determination of endotoxincore antibodies in serum, plasma and cell culture supernatant.

The EndoCAb® ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 2½ hours. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with equimolar amounts of endotoxin rough-lipopolysaccharides from four Gram-negative bacterial species, each comprised of a complete inner core, but lacking complete outer core or O-specific polysaccharide chain. Anti-endotoxin-core antibodies are captured by the solid phase antigen. Peroxidase conjugated anti-human antibody will bind to captured endotoxin-core antibodies. The peroxidase conjugated antibody will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the EndoCAb® standards (log). The endotoxin-core concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.

Product type
3 x 96 det.
Standard range
Standard curve of IgG from 0.13‑8 GMU/­ml. Standard curve of IgM from 0.05-3.5 MMU/ml. Standard curve of IgA from 0.16-10 AMU/ml.
Detection level
Minimum detection level IgG EndoCAb® antibodies is 0.13 GMU/ml. - Minimum detection level IgM EndoCAb® antibodies is 0.05 MMU/ml. - Minimum detection level IgA EndoCAb® antibodies is 0.16 AMU/ml.
Working volume
100 µl/well
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Gastroenterology, Infectious diseases
Assay Manual Safety Data Sheet

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