EndoCAb® IgA, Human, ELISA kit

The EndoCAb® IgA ELISA has been developed for determination of endotoxin core
antibodies in human plasma or serum in patients or healthy individuals. Several studies show
a consistent drop in postoperative levels of circulating anti-endotoxin core antibodies
compared to the preoperative value. This drop has been interpreted as consumption of
antibodies to endotoxin by systemic release of endotoxin. A hypothesis is that if the patients
pre-operative endotoxin-core level is low, even moderately low, patients may not be able to
cope with the efflux of endotoxin, which may have mild to severe clinical consequences. The
assay is of interest for various experimental conditions ranging from in vitro LPS
neutralization by plasma components to in vivo studies on kinetics of antibodies to endotoxin
in health and diseases.
The EndoCAb® standard median-units IgA (MU) are arbitrary and are based on medians of
ranges for 1000 healthy adults in a particular locality. It has not been established whether
normal endotoxin-core antibody values vary by region, culture or race. Users should
establish appropriate local statistical controls for their studies. It is advised that studies of any
patient group should always be controled by studies of appropriately selected contrasting
clinical groups and/or healthy individuals recruited locally.
EndoCAb® is a registered trademark. Used under license from the Scottish National Blood Transfusion
Service.