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Catalog # HM1021

TNF-alpha, Mouse, mAb V1q

120,00413,00

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The monoclonal antibody V1q recognizes mouse tumor necrosis factor alpha (TNF-α). TNF-α is the
prototype cytokine of the family of TNF-related ligands, which are based on structural and functional
homologies. TNF-α is synthesized as type II transmembrane protein. TNF-α can be recognized by two
different membrane receptors, namely TNF-R1 and TNF-R2. TNF-α is present in a membrane-bound
(tmTNF) as well as soluble form (sTNF). The membrane-bound form of TNF-α is recognized by both
TNF receptors with high affinity, whereas the soluble form is recognized more superiorly by TNF-R1.
TNF-α is produced by many different cell types including macrophages, T lymphocytes, NK cells,
neutrophils and endothelial cells. Cells differ in the expression of the two TNF-receptors and sTNF
versus tmTNF, respectively.
TNF-α, a homotrimeric 17 kDa protein, is a potent mediator of inflammatory and metabolic functions.
TNF-α was originally detected as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis
in vivo and shows cytolytic activity against tumor cells in vitro. Furthermore, TNF-α has been implied
as central mediator in shock induced by gram negative micro-organisms. TNF-α induces on its turn
the production of many other cytokines. Furthermore, TNF-α has been found in inflammatory foci such
as synovial effusions in rheumatoid arthritis, systemic circulation in septic shock, parasitemia and
rejection of renal transplants. The monoclonal antibody V1q recognizes both natural and recombinant
TNF-α and shows neutralizing activity.

Application
Flow cytometry, Functional studies

Application Notes
FC: Antibody V1q stains the extracellular domain of mouse TNF-;. The CHO cells were fixed in PBA containing 0.2 % formaldehyde before staining. As positive control mTNF-; transfected cells were used. (Ref.2)
FS: Antibody V1q functions as a neutralizing antibody. The antibody was functionally tested by neutralization of the cytopathic effect of cytotoxin in the L929 TNF bioassay. The biological activity of the antibody can be defined as the concentration of V1q required to neutralize 100 U/ml of TNF/cytotoxin. (Ref.1)

Use
For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.

Positive Control
Monocytes/macrophages

Product type
Monoclonal antibodies
Quantity
20 µg, 100 µg
Formulation
0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
Immunogen
Cytotoxin purified from conA induced T cell clone 29
Species
Mouse
Alias
TNF, TNF-SF2, DIF, cachectin, TNF-α, tumor necrosis factor ligand superfamily member 2
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Autoimmunity, Gastroenterology
Protocol CoA-TDS HM1000-HM1066
HM1021F
39 kb

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