TNF-RII, Human, mAb MR2-1, FITC

Name: TNF-RII, Human, mAb MR2-1, FITC
SKU: HM2007F
Availability: InStock

The antibody MR2-1 reacts with the extra-cellular part of the TNF-RII. It also reacts with the soluble receptor.

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Description
Application

The antibody MR2-1 reacts with the extra-cellular part of the TNF-RII. It also reacts with the soluble receptor.

TNF-RII is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-alpha. TNF-RII molecule is shown to be responsible for stimulation of activated T-lymphocytes by TNF-alpha. The antibody cross reacts with rhesus and cynomolgus natural TNF-RII.

Application

Flow cytometry, Functional studies, Immuno assays, Immuno fluorescence, Immuno precipitation, Western blot

Application Notes

IA: HM2007 can be used as a capture antibody.
IP: The lysate was centrifuged and the supernatant was incubated with 5 mg TNFR2-specific antibody MR2-1 for 2 hours at 4°C. The immuno complexes were precipitated with protein G agarose for two hours at 4°C (Ref.4).
W: A reduced protocol was used. The expected band size is ~65 kDa.

Use

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Specifications
Downloads

Product type

Monoclonal antibodies

Amount

100 µg, 20 µg

Formulation

0.2 ml (100 μg/ml) 0.2 μm filtered protein G purified FITC conjugated antibody solution in PBS, containing 1% bovine serum albumin and 0.02% sodium azide.

Conjugate

FITC

Immunogen

sTNF-R75 purified from the culture supernatant of NSO cells (transfected with the extracellular part of TNF-R75) (Ref.1).

Isotype

Mouse IgG1

Species

Human

Cross reactivity

Cynomolgus monkey – Yes, Rhesus monkey – Yes

Alias

Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, TNF-R2, Tumor necrosis factor receptor type II, TNFR-II, p75, p80 TNF-alpha receptor, CD120b<br> - Gene name: TNFRSF1B, TNFBR, TNFR2

Storage and stability

Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year.

Precautions

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hbt is not responsible for any patent infringements that might result from the use or derivation of this product.

Disease

Autoimmunity, Gastroenterology

Protocol