COMING SOON: C3bBbP, Human, ELISA kit

Immuno assays

The human C3bBbP ELISA kit is to be used for the in vitro quantitative determination of C3bBbP in serum and plasma samples.

The human C3bBbP ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 1 hour and 15 minutes. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing human C3bBbP. Peroxidase-conjugated antibody will bind to the captured clusterin. Peroxidase-conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human clusterin standards (log). The human clusterin concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.