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Box of Hycult Biotech ELISA kit
Catalog # HK3002

C3bBbP, Human, ELISA kit

The human C3bBbP ELISA kit is to be used for the in vitro quantitative determination of C3bBbP in serum and plasma samples.

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The complement system is a vital component of innate immunity, serving initially as the first line of defense against pathogens. It consists of three activation pathways: the classical, lectin, and alternative pathways, all converging at the activation of complement protein C3. The alternative pathway is unique in its ability to amplify immune responses via the formation of the C3 convertase complex, C3bBb, which plays a crucial role in pathogen clearance and immune surveillance.
C3bBbP, also known as the properdin-stabilized C3 convertase, is an extended form of C3bBb. Properdin, the only known positive regulator of complement activity, binds to C3bBb, increasing its stability and prolonging its function. This stabilization enhances the cleavage of C3 into C3a and C3b, leading to a powerful amplification loop that ensures rapid immune responses. However, dysregulation of this mechanism can contribute to pathological conditions.
Elevated levels of C3bBbP have been implicated in complement-mediated diseases, including C3 glomerulopathy (C3G) and atypical hemolytic uremic syndrome (aHUS). These disorders are characterized by excessive complement activation, leading to uncontrolled inflammation and tissue damage. Additionally, the presence of nephritic factors (autoantibodies that stabilize C3bBbP) can further disrupt complement homeostasis, exacerbating disease progression.
The detection of C3bBbP serves as a potential diagnostic biomarker for alternative pathway complement-associated diseases. Its measurement helps to assess alternative pathway activity, providing valuable insights into disease mechanisms and possibly guiding complement-targeted therapies. As complement-directed treatments continue to progress, the role of C3bBbP is becoming increasingly significant.

Application
Immuno assays

Use
The human C3bBbP ELISA kit is to be used for the in vitro quantitative determination of C3bBbP in serum and plasma samples.

Principle
The human C3bBbP ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 1 hour and 15 minutes. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing human C3bBbP. Peroxidase-conjugated antibody will bind to the captured clusterin. Peroxidase-conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human clusterin standards (log). The human clusterin concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.

Amount
1 x 96 det., 2 x 96 det.
Product type
Assays
Working volume
100 µl/well
Species
Cynomolgus Monkey, Human, Mouse
Alias
properdin-stabilized AP C3 convertase, C3bBbP complex
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Assay Manual
HK3002
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