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Catalog # HM3033

C5b-9, Rat, mAb 2A1

The monoclonal antibody 2A1 recognizes rat C5b-9.

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The monoclonal antibody 2A1 recognizes rat C5b-9 or TCC.

The antibody was shown to compete with antibodies to human C9 for its binding site on the C5b-9 complex, indicating that the reactive epitope is located on the C9 molecule. C5b-9 membrane attack complexes are assembled from five precursor molecules in the serum. Proteolytic cleavage of C5 by C5 convertase generates C5b which initiates assembly of the C5b-9 complex. The last step of C5b-9 complex formation involves polymerization of C9 which accompanies insertion of the complex into the cell membrane.

During formation of C5b-8 and C9 polymerization, neoantigens are generated which are unique to the C5b-9 complex and are not present on any of the individual native complex components. The complement regulatory proteins CD59 and complement S-protein can both prevent C5b-9 insertion into the cell membrane. The formed SC5b-9 complex is unable to attach to cells and is cytolytically inactive.C5b-9 is involved in the progression of chronic proteinuric renal disease by mediating continuous tubulointerstitial damage. Early tubulointerstitial injury in the remnant kidney can be improved when C5b-9 complex forming is abrogated.

The monoclonal antibody 2A1 was raised against a rat C5b-9 neoantigen. Monoclonal antibody 2A1 can be used as a coating antibody to detect C5b-9 in plasma and urine samples.

Flow cytometry, Frozen sections, Immuno assays, Immuno fluorescence, Paraffin sections

Application Notes
IA: plates were coated with 30μg/ml in 50mM carbonatebuffer, pH10.6 for 16h at 4°C.(Ref.1)
IHC-P: Tissue sections fixed in formalin were pretreated with protease type XXIV for 10 minutes at 37⁰C before incubation (Ref.6).
IHC-F: Tissue sections were fixed in acetone for 10 minutes at room temperature before incubation (Ref. 3).

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Positive Control
Glomeruli of rats treated with anti-Thy-1.1 antibodies

Product type
Monoclonal antibodies
100 µg, 20 µg, 500 µg
Quantities >500 µg will be send without preservative and carrier free. Quantities <500 µg will be send with preservative and carrier. exact formulation can be found on the datasheet.
Rat C5b-9
Mouse IgG1
Membrane attack complex, MAC, TCC, Terminal Complement Complex
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Infectious diseases, Nephrology

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