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Catalog # HM3034

C6, Rat, mAb 3G11

The monoclonal antibody 3G11 recognizes rat C6. Complement component C6 is a key part of the C5b-9 membrane attack complex (MAC/TCC).

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133.00368.00

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Monoclonal antibody 3G11 for detection of rat C6.

This monoclonal antibody clone 3G11 recognizes rat C6. Complement component C6 is a key part of the C5b-9 membrane attack complex (MAC/TCC).

In serum, five precursor molecules cooperate to assemble C5b-9 membrane attack complexes. First, C5 convertase cleaves C5, forming C5b, which then triggers C5b-9 complex formation. Subsequently, in the final step, C9 polymerizes, enabling the complex to insert into the cell membrane. Simultaneously, during C5b-8 formation and C9 polymerization, specific neoantigens emerge that are not present on native complex components.

The rat remnant kidney model has been widely used to study mechanisms underlying renal disease progression. Proteinuria demonstrates nephrotoxic properties and contributes to renal injury. Complement activation in proteinuric urine results in tubular and interstitial damage. However, in C6-deficient rats, acute interstitial changes resolved despite persistent proteinuria. This finding suggests that C6 plays an important role in mediating chronic tubulointerstitial damage in remnant kidney models.

To address this, the monoclonal antibody 3G11 was specifically developed by immunizing mice with C6 isolated from rat serum. Due to its specificity, this antibody serves as a reliable antibody for detecting C6 in both serum and urine samples antibody in immunoassays to measure C5b-9 in both serum and urine samples.

You may also find monoclonal antibody HM3033 clone 2A1 which recognizes rat C5b-9, to be of interest.

Application
Frozen sections, Immuno assays, Western blot

Use
For immunohistology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
Amount
100 µg, 20 µg
Formulation
0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Isotype
Mouse IgG1
Species
Rat
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Infectious diseases, Nephrology, Neurological disorders
CoA-TDS Safety Data Sheet
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