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Catalog # HM3034

C6, Rat, mAb 3G11

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The monoclonal antibody 3G11 reacts specifically with rat C6. Proteolytic cleavage of C5 by C5 convertase generates C5b which initiates assembly of the C5b-9 membrane attack complex. Complement component C6 is part of this C5b-9 complex. This complex is assembled from five precursor molecules in the serum. The final step of C5b-9 complex formation involves polymerization of C9 which accompanies insertion of the complex into the cell membrane. The rat remnant kidney model had been widely used to identify mechanisms responsible for the progression of renal disease. Proteinuria is nephrotoxic and contributes to the progression of renal injury. Activation of complement in proteinuric urine results in tubular and interstitial damage. In C6-deficient rats, acute interstitial changes resolved despite ongoing proteinuria. This suggests that C6 mediates chronic progression of tubulointerstitial damage in rats with remnant kidneys. The monoclonal antibody 3G11 was raised by immunization of mice with C6 isolated from rat serum. Monoclonal antibody 3G11 can be used as detector in immuno assays detect C5b-9 in both serum and urine.

Frozen sections, Immuno assays, Western blot

For immunohistology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
100 µg, 20 µg
0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Mouse IgG1
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Infectious diseases, Nephrology

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