C6, Rat, mAb 3G11

Monoclonal antibody 3G11 for detection of rat C6.

This monoclonal antibody clone 3G11 recognizes rat C6. Complement component C6 is a key part of the C5b-9 membrane attack complex (MAC/TCC).

In serum, five precursor molecules cooperate to assemble C5b-9 membrane attack complexes. First, C5 convertase cleaves C5, forming C5b, which then triggers C5b-9 complex formation. Subsequently, in the final step, C9 polymerizes, enabling the complex to insert into the cell membrane. Simultaneously, during C5b-8 formation and C9 polymerization, specific neoantigens emerge that are not present on native complex components.

The rat remnant kidney model has been widely used to study mechanisms underlying renal disease progression. Proteinuria demonstrates nephrotoxic properties and contributes to renal injury. Complement activation in proteinuric urine results in tubular and interstitial damage. However, in C6-deficient rats, acute interstitial changes resolved despite persistent proteinuria. This finding suggests that C6 plays an important role in mediating chronic tubulointerstitial damage in remnant kidney models.

To address this, the monoclonal antibody 3G11 was specifically developed by immunizing mice with C6 isolated from rat serum. Due to its specificity, this antibody serves as a reliable antibody for detecting C6 in both serum and urine samples antibody in immunoassays to measure C5b-9 in both serum and urine samples.

You may also find monoclonal antibody HM3033 clone 2A1 which recognizes rat C5b-9, to be of interest.