Complement factor H, Human, ELISA kit

Name: Complement factor H, Human, ELISA kit
SKU: HK342
Availability: InStock

Complement Factor H (CFH), an essential protein in the complement system, plays a crucial role in immune defense, inflammation moderation, and immune complex management.

Please choose the quantity of items you wish to add to your cart…

Find distributor

Complement Factor H (CFH) is key in the complement system, vital for immune defense and inflammation control. With a robust presence in the bloodstream at 400-800 µg/ml, CFH stabilizes complement activity, targeting immune responses. It binds C3b, speeding up alternative pathway C3-convertase breakdown and aiding C3b deactivation, thereby shielding the body from undue tissue damage.

CFH’s structure, a 150 kDa glycoprotein with 20 consensus repeats, is crucial for its function. These repeats have sites essential for CFH’s anti-inflammatory role and CRP interaction, affecting cell signaling. CFH’s N-terminal and C-terminal domains, notably SCR 7, are key in fluid regulation and cellular defense. The link between CFH gene variations and a spectrum of human diseases, including kidney disorders like atypical Hemolytic Uremic Syndrome (aHUS) and vision-threatening conditions like age-related macular degeneration (AMD), underscore its clinical relevance and the potential for targeted CFH-based therapies.

Application

The human complement factor H ELISA kit is to be used for the in vitro quantitative determination of human complement factor H in serum, plasma and urine samples.

Principle

The human complement factor H ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 2½ hours. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing human complement factor H. Peroxidase conjugate antibody will bind to the captured human complement factor H. Peroxidase conjugate antibody will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human complement factor H standards (log). The human complement factor H concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.

Recovery

Normal human blood samples (plasma), containing baseline levels of human complement factor H, were spiked with human complement factor H in concentrations of 62.5 and 15.6 ng/ml. Samples with and without human complement factor H were incubated for 1 hour at room temperature. Samples were measured using the ELISA. Values for human complement factor H ranged between 65% and 134% (mean 102%).

Specifications
Downloads

Product type

Assays

Amount

1 x 96 det., 2 x 96 det.

Standard range

3.9 – 250 ng/ml

Detection level

3.9 ng/ml

Working volume

100 µl/well

Species

Human

Storage and stability

Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.

Precautions

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.

Disease

Autoimmunity, Nephrology

Safety Data Sheet