Properdin, Human, ELISA kit

The human Properdin ELISA kit is to be used for the in vitro quantitative determination of human Properdin in serum, plasma, bronchoalveolar lavage fluid, urine and cerebrospinal fluid samples.

The human Properdin ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing human Properdin. Biotinylated tracer antibody will bind to the captured human Properdin. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human Properdin standards (log). The human Properdin concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.

The linearity of the assay was determined by serially diluting a serum. The line obtained a correlation coefficient of 0.9966

Normal human blood samples (plasma) containing baseline levels of human Properdin, were spiked with human Properdin, in concentrations of 500 and 2000 ng/ml. Samples with and without human Properdin, were incubated for 1 hour at room temperature. Samples were measured using the ELISA. Values for human Properdin, ranged between 85.1% and 115.3%.