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Catalog # HK3004

FHR-2, Human, ELISA kit

The human FHR-2 ELISA kit is to be used for the in vitro quantitative determination of FHR-2 in serum and plasma samples.

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Complement Factor H-Related Protein 2 (FHR-2) is a significant member of the complement regulatory protein family, with a molecular weight of around 29 kDa. Synthesized mainly in the liver, FHR-2 is also produced by immune cells like monocytes, macrophages, and dendritic cells. Sharing structural homology with Complement Factor H (CFH), FHR-2 plays a role in regulating the complement cascade, although its exact biological function remains somewhat elusive and subject to debate.
Research indicates that FHR-2 may inhibit the activity of the alternative pathway (AP) C3 convertase, which would theoretically prevent the formation of the membrane attack complex (MAC). Conversely, some studies suggest that FHR-2 might actually promote AP activation, highlighted by findings where a hybrid mutant protein comprising FHR-5 domains and full-length FHR-2 led to overactivation of the complement system.
FHR-2 has been linked to various diseases where abnormal complement activation is a contributing factor. Particularly, it is associated with atypical hemolytic uremic syndrome (aHUS) and age-related macular degeneration (AMD), where dysregulation in complement activation leads to detrimental tissue deposition. The involvement of FHR-2 in these pathologies, specifically in the alternative pathway, makes it a potential therapeutic target in managing diseases driven by complement dysregulation.
FHR-2 can be measured with our HK3004 assay, designed to precisely quantify this intriguing glycoprotein.

Immuno assays

The human FHR-2 ELISA kit is to be used for the in vitro quantitative determination of FHR-2 in serum and plasma samples.

The human FHR-2 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 1 hour and 15 minutes. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing human FHR-2. Peroxidase-conjugated antibody will bind to the captured FHR-2. Peroxidase-conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human FHR-2 standards (log). The human FHR-2 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.

1 x 96 det., 2 x 96 det.
Product type
Working volume
100 µl/well
Cross reactivity
Monkey – Yes, Mouse – No, Pig – No, Rat – No
Complement Factor H-related protein 2
Autoimmunity, Infectious diseases, Nephrology, Tumor immunology
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Assay Manual
567 kb
Safety Data Sheet Folders

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