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Catalog # HK3007

FHR-5, Human, ELISA kit

The human FHR-5 ELISA kit is to be used for the in vitro quantitative determination of FHR-5 in serum and plasma samples.

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Complement Factor H-related protein 5 (FHR-5) is a vital glycoprotein in the complement regulatory protein family. Predominantly synthesized in the liver, FHR-5, Human, also sees production from monocytes, macrophages, and dendritic cells. Weighing approximately 65 kDa, it structurally resembles Complement Factor H (CFH), a crucial regulator in the complement system. FHR-5’s primary role lies within the alternative pathway (AP) of the complement system.
FHR-5’s functionality extends to binding with various host ligands, including C3b, heparin, C-reactive protein (CRP), Pentraxin 3 (PTX3), and elements of the extracellular matrix. It actively promotes alternative pathway activation on cell surfaces, offering a scaffold for AP C3 convertase assembly. Moreover, FHR-5 may challenge Factor H (FH) by competing for ligand binding, potentially influencing FH’s regulatory activities.
Disease associations of FHR-5 are significant yet complex. Genetic variations in the CFHR5 gene have been identified in patients with atypical hemolytic uremic syndrome (aHUS) and age-related macular degeneration (AMD). Furthermore, its presence in both circulating and glomerular forms is linked to IgA nephropathy (IgAN) and familial C3 glomerulopathy (C3G). However, the specific role of FHR-5 in these conditions remains under debate, and other genetic factors may also contribute, underscoring the necessity for further research in this area.
FHR-5, Human, can be measured with our HK3007 assay, designed to precisely quantify this intriguing glycoprotein.

Immuno assays

The human FHR-5 ELISA kit is to be used for the in vitro quantitative determination of FHR-5 in serum and plasma samples.

The human FHR-5 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 1 hour and 15 minutes. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing human FHR-5. Peroxidase-conjugated antibody will bind to the captured FHR-5. Peroxidase-conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human FHR-5 standards (log). The human FHR-5 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.

1 x 96 det., 2 x 96 det.
Product type
Working volume
100 µl/well
Cross reactivity
Monkey – No, Mouse – No, Pig – No, Rat – No
Complement Factor H-related protein 5
Autoimmunity, Infectious diseases, Nephrology, Tumor immunology
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Assay Manual
472 kb
Safety Data Sheet Folders

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