Home \ Antibodies \ Ficolin-2, Human, mAb GN5
Catalog # HM2091

Ficolin-2, Human, mAb GN5

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The monoclonal antibody GN5 recognizes human Ficolin-2, also known as L-ficolin. Ficolins are a family of pattern recognition molecules containing both a collagen-like domain and a fibrinogen-like domain that are capable of activating the complement system, through association with MASPs, on the surface of microorganisms. In human, three Ficolins are known: Ficolin-1 (M-ficolin), Ficolin-2 (L-ficolin), and Ficolin-3 (H-ficolin). Ficolin-1, is mainly expressed by monocytes, granulocytes and lung cells and is present locally in areas of inflammation.
Ficolin-2/P35 (also L-ficolin, ficolin L, EBP-37 or hucolin) is produced primarily by the liver. The association between Ficolin-2/P35 and MASPs/sMAP is Ca2+-dependent. MASPs in Ficolin-2/P35 -MASP complexes have proteolytic activities for C4, C2 and C3. Ficolin-2/P35 also shows opsonic activity.

Application
Frozen sections, Immuno assays, Immuno precipitation, Western blot

Application Notes
W: A non-reduced sample treatment and SDS-Page was used. The band size is 35 kDa.
IHC-F: Tissue sections were fixed in acetone and pretreated with peroxidase block of the Dako kit 5 min. in the dark at RT to quench endogenous peroxidases.

Use
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
Amount
100 µg, 20 µg, 500 µg
Formulation
Quantities >500 µg will be send without preservative and carrier free. Quantities <500 µg will be send with preservative and carrier. exact formulation can be found on the datasheet.
Isotype
Mouse IgG1
Species
Human
Cross reactivity
Rat – Yes
Alias
Ficolin-2/P35, L-ficolin, ficolin L, EBP-37, hucolin, 37 kDa elastin-binding protein, Collagen/fibrinogen domain-containing protein 2, Ficolin-B, Ficolin-beta</br> - Gene name: FCN2, FCNL
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease
Cardiology and metabolism, Infectious diseases

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