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Catalog # HK323

MBL, Human, ELISA kit

Mannose Binding Lectin (MBL), a crucial C-type lectin in innate immunity, stands out in the collectin protein family with its unique collagen-like and carbohydrate recognition domains.

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713.001,153.00

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Mannose Binding Lectin (MBL), an integral component of innate immunity, belongs to the collectin protein family. It uniquely combines a collagen-like domain with a carbohydrate recognition domain (CRD). This structure enables MBL to recognize specific carbohydrates, such as mannose and N-acetylglucosamine, present on pathogens. MBL forms oligomers, each comprising three identical 32 kDa polypeptides, functioning as a pattern recognition receptor. Upon encountering infectious agents, MBL activates the lectin-complement pathway, independent of the classical and alternative pathways.

In this pathway, the MBL-MASP complex crucially cleaves C4, C2, and C3. Unlike the classical and alternative pathways, it operates without the need for antibodies and C1q. Primarily synthesized in the liver, MBL is also present in the serum of various vertebrate species. In human plasma, MBL levels range significantly, from 10 to 5,000 ng/ml. Notably, up to 12% of healthy Caucasians exhibit MBL concentrations below 100 ng/ml, often associated with inherited opsonization defects.

MBL levels rise in response to infectious diseases, making its measurement essential in various medical scenarios. These include recurrent infections in children, immunodeficiencies, cardiovascular diseases, cystic fibrosis, and autoimmune conditions. The human MBL assay, designed for precision, remains unaffected by antibodies against mannan. Utilizing a specialized binding buffer, it inhibits classical pathway interference while preserving MBL’s functionality. This approach underscores MBL’s critical role in immune system diagnostics and research.

Application
The human MBL (Lectin assay) ELISA kit is to be used for the in vitro quantitative determination of human MBL in serum, plasma and cell culture supernatant samples.

Principle
The human MBL ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 4 hours. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. After activation, samples and standards are captured by solid bound mannan. Biotinylated tracer antibody will bind to captured MBL. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the MBL standards (log). The MBL concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.

Product type
Assays
Amount
1 x 96 det., 2 x 96 det.
Standard range
0.41-100 ng/ml
Detection level
0.41 ng/ml
Working volume
100 µl/well
Species
Human
Alias
Mannose- or mannan-binding protein (MBP)
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease
Infectious diseases
Cross reactivity
Cynomolgus monkey – Yes, Pig – No
Safety Data Sheet Assay Manual
HK323
399 kb

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