Home \ Antibodies \ S100A4, Human, mAb 5A3
IHC-P: BxPC3 tumor section
Catalog # HM2334

S100A4, Human, mAb 5A3

413,001.283,00

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The monoclonal antibody clone 5A3 recognizes S100A4. S100A4 is a member of the S100 family. To date, the family consists of 22 members. S100 proteins are low molecular weight calcium binding proteins. The proteins consist of 2 calcium binding EF-hands located on the termini flanked by hydrophobic hinge regions. Calcium ions acts as a global second messenger involved in the regulation of many aspects of cell function. S100 proteins are involved in sensing and transforming calcium signals to downstream cellular responses. S100 proteins are located in the cytoplasm and/or nucleus of a wide range of cell types and involved in the regulation of cellular processes such as cell cycle progression and differentiation and inflammatory responses. Several S100 members have been found to act as cytokines in inflammation, particularly in autoimmune skin conditions such as psoriasis. S100A4 may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered S100A4 gene expression have been implicated in tumor metastasis. Studies have shown that S100A4 is involved tumor progression and metastasis in various malignant tumors. Detection of S100A4 expression becomes a promising candidate biomarker in cancer early diagnosis and prediction of cancer metastasis. Therefore S100A4 may have high therapeutic value.

Application
Functional studies, Immuno assays, Paraffin sections, Western blot

Application Notes
W: A reduced sample treatment and SDS-Page was used. The band size is ~12 kDa.
FS: Antibody clone 5A3 can be used as blocking antibody.

Use
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
Quantity
20 µg, 100 µg, 500 µg
Formulation
Quantities >500 µg will be send without preservative and carrier free. Quantities <500 µg will be send with preservative and carrier. exact formulation can be found on the datasheet.
Immunogen
Full length human recombinant protein isolated from E.coli.
Isotype
Mouse IgG1
Species
Human
Cross reactivity
Cat – Yes, Dog – Yes, Rabbit – Yes, Sheep – Yes
Alias
Protein S100-A4, Calvasculin, Metastasin, Placental calcium-binding protein, Protein Mts1, S100 calcium-binding protein A4
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Tumor immunology
CoA-TDS HM2300-HM2400
HM2334
84 kb

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