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Catalog # HP6001

SbmA, pAb

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This polyclonal antibody recognizes the inner membrane protein SbmA of Gram-negative bacteria. The SbmA protein is a homodimeric secondary transporter protein, that is involved in the transport of microcins B17 and J25, glycopeptide antibiotic bleomycin, proline-rich antimicrobial peptides of prokaryotic and eukaryotic origin, antisense peptide phosphodiamidate morpholino oligomers and peptide nucleic acids (PNA) into the E.coli cytoplasm. This protein is found in a large number of Gram-negative and also Gram-positive microorganisms, including plant and animal pathogens. The monomeric SbmA protein contains 406 amino acids (about 50 kDa) and has 8 transmembrane regions. The homodimeric complex present in the membrane closely resembles the membrane-spanning region of the ATP-binding cassette transporter family. Peptide transport mediated by SmbA is driven by a electrochemical gradient. SbmA-mediated internalization of peptide substrates suggest that the transport of an unknown substrate(s) represents the function of this protein.

Application
Immuno precipitation, Western blot

Application Notes
W: The band size to be expected is ~37 kDa under reducing conditions.

Use
For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Positive Control
Recombinant SbmA or E.coli lysate

Product type
Polyclonal antibodies
Amount
100 µg, 20 µg
Formulation
0.2 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Immunogen
Recombinant SbmA
Isotype
Rabbit Ig
Species
N/A
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Infectious diseases
Protocol
Protocol Immuno precipitation
54 kb
Protocol Western Blot
59 kb
CoA-TDS pAb
HP6001
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HP6001-20UG
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Safety Data Sheet
SDS - HP6001-100UG
52 kb
SDS - HP6001-20UG
52 kb

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