Home \ Antibodies \ PECAM-1, Mouse, mAb MEC7.46
HM1013 bENd3 cells 10µg/ml
Catalog # HM1013

PECAM-1, Mouse, mAb MEC7.46

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The monoclonal antibody MEC7.46 recognizes the mouse form of the platelet-endothelial cell adhesion molecule (PECAM)-1 (CD31). PECAM-1 is a member of the immunoglobulin superfamily. This heavily glycosylated protein is found in the entire vascular endothelium of adult mice and functions in mediating cellular adhesion by heterophilic and homophilic mechanisms. PECAM-1 is detected within the lymphopoietic islands in the spleen of newborn (day 12) and in the bone marrow of adult mice. Capillary endothelial cells of adult mice also express PECAM-1.
The reactivity of the monoclonal antibody MEC7.46 is restricted to the isoform of the molecule that is selectively expressed by endothelial cells. The antibody precipitates a 130 kDa molecule present on the membrane of endothelial cells of all mouse blood vessels both in normal, inflamed and tumor tissues. The antigen is predominantly present at the lateral borders of endothelial cells as described for human PECAM-1. Staining of MEC7.46 can be seen on capillaries, veins, arteries and liver sinusoids.

Application
Flow cytometry, Frozen sections, Immuno fluorescence, Immuno precipitation, Paraffin sections

Application Notes
FC: Antibody MEC7.46 stains the extracellular domain of mouse PECAM-1; 106 cells were used per sample. No fixation or permeabilization needed. Epitope is sensitive to trypsin! (Ref.1)
F: Tissue sections were fixed in acetone for 10 min at RT and pretreated with 0.01% hydrogen peroxide to quench endogenous peroxidases. Normal rabbit serum was used as blocking agent. As (Ref.1).
P: Serial paraffin-embedded 4 μm sections were stained by H&E, PAS and Azan Histochemistry. The distribution of endothelial (Mec7.46), matrix (laminin) and macrophage (FA/11) markers was evaluated using a standard three-step ABC method and development in 3-amino-9-ethylcarbazole solution. Sections were counterstained for 45 seconds with Harris' haematoxylin at room temperature and mounted in Imsol-mount medium (ref. 3).
IP: Lysate of 35S-Methionine labeled 5HV cells was used. Molecule of ~130 kDa.

Use
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Positive Control
Mouse t-end.1 cells

Negative Control
L929 cells (Fibroblasts)

Product type
Monoclonal antibodies
Amount
100 µg, 20 µg
Formulation
0.2 ml (100 μg/ml) 0.2 μm filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Immunogen
Mouse t.end.1 cells (polyoma middle T (PmT)-transformed EC)
Isotype
Rat IgG1
Species
Mouse
Alias
CD31, platelet-endothelial cell adhesion molecule-1 </br> - Gene name: Pecam1
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Cardiology and metabolism
Protocol
Protocol Immuno precipitation
54 kb
Protocol Immuno Fluorescence
39 kb
Protocol Flow Cytometry
58 kb
Protocol IHC-F
36 kb
CoA-TDS HM1000-HM1066
HM1013-100UG
96 kb
HM1013-20UG
95 kb
HM1013BT-50UG
95 kb
HM1013F-100UG
95 kb
HM1013F-20UG
96 kb
Safety Data Sheet
SDS - HM1013-20UG
52 kb
SDS - HM1013-100UG
52 kb

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