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Catalog # HM1024

IL-1RI, Mouse, mAb D1f3

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The monoclonal antibody binds to soluble mouse interleukin-1 receptor type I. The IL-1 system includes two agonists (IL-1alpha and IL-1beta), converting enzymes, antagonists, two receptors (IL-1RI and IL-1RII) and the IL-1 receptor accessory protein. Interleukin-1 signal is transduced through the type I receptor.

Application
Functional studies, Western blot

Application Notes
The antibody can be used for Western blotting. Furthermore the antibody is useful for inhibition in biological assays, in vitro as well as in vivo. Although for in vitro use the monoclonal antibody HM1022 (clone Reg21) is recommended.

Use
For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For neutralization of biological activity dilutions have to be made according to the amounts IL-1 RI to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.

Product type
Monoclonal antibodies
Amount
100 µg, 20 µg
Formulation
0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Isotype
Rat IgG1
Species
Mouse
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease
Autoimmunity, Neurological disorders
Protocol
Protocol Western Blot
59 kb
Protocol SDS Page
57 kb
CoA-TDS HM1000-HM1066
HM1024-100UG
51 kb
HM1024-20UG
51 kb
Safety Data Sheet
SDS - HM1024-100UG
51 kb
SDS - HM1024-20UG
51 kb

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