Home \ Antibodies \ Villin-1, Human, mAb 9H11
Catalog # HM2442

Villin-1, Human, mAb 9H11

This monoclonal is unique, since it is not cross reactive with any of the seven proteins that belong to the Factor H (FH)-protein family.

 

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125.00390.00

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The Villin-1 monoclonal antibody, HM2442 clone 9H11, targets human villin-1, a cytoskeletal protein of 92.5 kDa found in the microvilli of intestinal epithelium enterocytes and kidney proximal tubular cells. As a critical component of cellular architecture, villin-1’s presence in bodily fluids like blood and urine is indicative of cellular restructuring or damage. Notably, the detection of villin-1 in patients with acute kidney injury (AKI) underscores its potential as a biomarker for renal health, with levels notably responding to treatments like necrostatin-1 during ischemia-reperfusion events.
Beyond renal implications, villin-1’s expression profiles contribute to prognostic evaluations in diseases such as hepatocellular carcinoma. This enhances its profile as a multifaceted marker. Its role extends to gastroenterology, where it acts as a mucosal integrity indicator. This is crucial for diagnosing ischemic intestinal damage commonly associated with necrotizing enterocolitis.
The HM2442 clone 9H11 is adepted for a variety of laboratory techniques, including immunohistochemistry (IHC), enzyme-linked immunosorbent assays (ELISA), and Western blot applications. It makes it an invaluable tool for researchers exploring villin-1’s role in health and disease.

Application
Frozen sections, Paraffin sections, Western blot

Application Notes
IHC: Human gut villi Tissue staining with HM2442. The slices were dried overnight in an oven at 60°C. Afterwards rehydrated with xylene, ethanol and water. Antigen retrieval was applied for 20 minutes with a sodium citrate buffer in an autoclave for 120 °C and washed afterwards with PBS. The blocking was performed with peroxidase blocking and avidin/biotin blocking kit. HM2442 was applied with a dilution of 1:200 and incubated for 30 minutes and washed. As a secondary antibody a horse anti mouse was applied at a dilution of 1:200 and incubated for 30 minutes and washed. The Vecatastain ABC kit was applied and incubated for 1 hour. Afterwards a DAB incubation was performed for about 45 seconds, add PBS to stop reaction. A Haematoxylin staining was performed. Then the slices were dehydrated with the help of ethanol and xylene. WB: A non-reduced sample treatment and SDS-Page was used. The band sizes visible around ~55 kDa and ~93 kDa.

Use
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
Amount
100 µg, 20 µg
Formulation
100 µg/ml – 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Immunogen
Human Villin-1
Isotype
Mouse IgG2b
Species
Human
Alias
VIL1; D2S1471
Gene
Gene name VIL1 Entrez Gene ID 7429 Uniprot P09237
Disease
Gastroenterology
Country of Manufacture
NL
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
CoA-TDS Safety Data Sheet

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